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Oligo Synthesis

Oligo Synthesis : CEPs

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Spacer Phosphoramidite 18

Spacer Phosphoramidite 18

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1918-02Spacer Phosphoramidite 18 0.25g £218.00 Quantity Add to Order
10-1918-90Spacer Phosphoramidite 18 100µmoles £86.00 Quantity Add to Order

Description

Spacer Phosphoramidite 18

Structure

 

Catalog Number: 10-1918-xx

Description: Spacer Phosphoramidite 18

18-O-Dimethoxytritylhexaethyleneglycol,1-[(2-cyanoethyl)-
(N,N-diisopropyl)]-phosphoramidite
Formula: C42H61N2O10P M.W.: 784.93 F.W.: 344.30

Diluent: Anhydrous Acetonitrile
Add fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/Technical/TB_ABITransfer.pdf.
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 2-3 days

SPACER MODIFIERS

The spacer phosphoramidites C3, 9, C12 and 18 are used to insert a spacer arm in an oligonucleotide. The compounds may be added in multiple additions when a longer spacer is required. 3’-Spacer C3 CPG may also act as a blocker of exonuclease and polymerase activity at the 3’-terminus. dSpacer is used to introduce a stable abasic site within an oligonucleotide. PC Spacer is a photocleavable C3 spacer modifier, part of our line of photocleavable (PC) modifiers

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Protocols

Material Safety Data Sheet

Glen Report 7.1: NEW MODIFICATION REAGENTS AND SUPPORTS

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Applications & Benefits

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1918-02 0.25grams .25grams 3.18 92.67 55.6 34.75 25.27 18.53 4.63
10-1918-90 100µmoles .078grams 1 20 12 7.5 5.45 4 1
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm  
Approximate Number of Additions
10-1918-02 0.25grams .25grams 4.75 .07 88.6 55.38 40.27 5.54  
10-1918-90 100µmoles .078grams 1.5 .07 23.6 14.75 10.73 1.48  
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm    
Approximate Number of Additions
10-1918-02 0.25grams .25grams 4.75 .07 90.2 56.38 41    
10-1918-90 100µmoles .078grams 1.5 .07 25.2 15.75 11.45  

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