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Cloning Kits and Related Enzymes

Cloning Kits and Related Enzymes

Cloning Kits and Related Enzymes

Cloning Kits and Related Enzymes

Intact Genomics develops and manufactures high-quality cloning kits and enzymes for a wide range of molecular cloning applications. Our portfolio includes the proprietary Quick10™ and ig-Fusion™ Cloning Kits, DNA ligases, exonucleases, DNA-modifying enzymes, and lambda packaging extracts. These products support rapid DNA assembly, seamless cloning, ligation, end repair, mutagenesis, library construction, and other specialized workflows—helping researchers achieve efficient, accurate, and consistent results.

 

Product

Size Available

Application & Benefits

Tth DNA Ligase

1,000 & 10,000 Units

Ideal for high-temperature, high-precision ligation assays, including SNP genotyping, mutation detection, LCR, NGS library preparation, and synthetic biology applications.

Taq DNA Ligase

10,000 Units

Ideal for routine ligation-based assays in cost-sensitive workflows, including SNP genotyping, mutation detection, and LCR.

T4 DNA Ligase

100,000 & 400,000 Units

Ideal for general DNA ligation, including cloning, vector construction, sticky-end and blunt-end ligation, and NGS library preparation.

T4 Polynucleotide Kinase (PNK)

2,500 Units

Used for DNA/RNA end modification, including 5′-end phosphorylation, dephosphorylation (3′-phosphatase activity), labeling, and NGS library preparation.

T4 DNA Polymerase

500 Units

Used for DNA end repair, blunting of overhangs, second-strand synthesis, and NGS library preparation.

IG® Lambda Packaging Extract

6 & 24 Reactions

High-efficiency in vitro packaging of lambda, cosmid, and fosmid DNA, achieving up to 1 × 10? pfu/µg DNA.

Quick10™ Cloning Kit

6 Reactions

Enables assembly-to-plate cloning in as little as 10 minutes.

ig-Fusion™ Cloning Kit

10, 50 & 100 Reactions

Clone any PCR product(s) into any linearized expression vector at any site using fusion cloning technology.

ig-Fusion™ Cloning Enzyme Premix

10, 50 & 100 Reactions

Standalone ig-Fusion™ enzyme premix for custom cloning workflows.

T5 Exonuclease

5,000 Units

Commonly used for DNA assembly, seamless cloning, homologous recombination, and generation of single-stranded overhangs.

Exonuclease III (Exo III)

10,000 Units, 25,000 Units

Used for controlled 3′→5′ digestion of double-stranded DNA in deletion mapping, DNA end polishing, and site-directed mutagenesis.