TAMRA NHS Ester - (50-5910) Carboxytetramethylrhodamine, N-hydroxysuccinimide ester in dimethyl sulfoxide (DMSO) |
Product Specifications
Formula:
C29H25N3O7 in DMSO
Rhodamine
derivatives are not sufficiently stable to survive conventional
deprotection and these must be attached to amino-modified
oligonucleotides using post-synthesis labelling techniques. Because
Tetramethyl Rhodamine (TAMRA) is not base stable, the procedure to
cleave and deprotect the labelled oligonucleotide must be carefully
considered. Using the UltraMILD monomers and deprotection with potassium
carbonate in methanol, TAMRA oligonucleotides can be fairly
conveniently isolated. To streamline the preparation of TAMRA oligos, we
offer 3'-TAMRA CPG for 3' labelling and TAMRA-dT for labelling within
the sequence. We also offer TAMRA NHS ester for labelling amino-modified
oligonucleotides.
The spectral characteristics of this dye is detailed here.
Details
Usage
- Coupling: Conjugate with an amino-modified oligo in sodium carbonate/bicarbonate buffer (pH=9). See Technical Bulletin for details.
- Deprotection: not applicable
| Specifications |
|---|
| Diluent | ~0.17M Solution in Anhydrous DMSO |
| Recommended Storage | Freezer storage, -10 to -30°C, dry |
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