Cambio - Excellence in Molecular Biology

In Vitro Toxicology

In Vitro Toxicology: Mutagenicity kits

The Ames II / Ames MPF Microfluctuation Assays are all modifications of the traditional Ames plate incorporation assay.

The Ames II assay uses the S. typhimurium strains TA98 and TAMix (a mixture of 6 strains, each detecting a specific base-pair substitution), whereas the Ames MPF® kits are available with the strains TA98, TA100, TA1535, TA1537, E.coli WP2 uvrA or E.coli WP2 pKM101.

 

Benefits of Xenometrix Ames Mutagenicity Assays over traditional Ames Procedures

  • Easy, compact and high throughput liquid format with 384 well plates.

  • Simple detection method with easy "readout" (as compared to the traditional assay)

  • Kits include all five types strains cited in OECD 471 (CoA supplied), quality controlled according to the same guideline 

  • Much less  test compound required.  (10 mg per strain if tested in triplicates, +/- S9, 6 semi-logarithmic compound dilutions)

  • Ames MPF is the preferred system for genotoxic impurities due to its low consumption of compound

  • Significantly less consumption of S9

  • Less environmental pollution due to significantly less "contaminated" waste

  • Less user intervention

  • Download presentation 

Ames MPF 98/100 Test Kits -with semi-solid strains

Rapid Screening: The Ames MPF 98/100 assay comprises the histidine auxotrophic Salmonella tester strains TA98 for detecting frameshift mutations.

Xenometrix AG

Catalogue No.DescriptionPack SizePriceQty
XA01-210-S2-PAmes MPF 98/100 -lyophilized rat liver S9, Phenobarbital/ß-Naphtoflavone induced Pos. Control 1 test £628.00 Quantity Add to Order
XA10-210Ames MPF 98/100 - 10 Sample Kit - 480 Measuring Points / Strain10 sample kit £2,083.00 Quantity Add to Order
XA10-210-S2-PAmes MPF 98/100 -lyophilized rat liver S9, Phenobarbital/ß-Naphtoflavone induced Pos. Control 10 tests £2,586.00 Quantity Add to Order

Description

Rapid Screening:
The Ames MPF 98/100 assay comprises the histidine auxotrophic Salmonella tester strains TA98 for detecting frameshift mutations and TA100 which is reverted by base-pair substitutions and all media required to perform the assay. Kits including additional S9 and Positive Controls are also available. TA98 and TA100 are commonly used for mutagenicity screening because they indicate both frameshift and base-pair

Kit contents

(Depending on kit size)

1 or 10 Vials semisolid TA98
1 or 10 Vials semisolid TA100
1 or 10 Vials Ampicillin
Growth -, Exposure - and Reversion Indicator Media
All media and strains provided are sufficient to analyze at least 1 or 10 compounds,
triplicates, 6 concentrations, negative and positive control, /- S9

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