Lab Reagents: Smart Buffers and Reagents
Biological buffers for the life science laboratory need to meet a wide array of criteria. These include good stability, lack of toxicity, precision in pH, pKa between 6.0 and 8.0 (the region in which most biological reactions occur), minimal salt effects due to the ionic composition of the solution, free of enzymatic and hydrolytic activity and minimal participation in biological reactions.
Buffers for molecular biology applications, e.g. Tris-Borate-EDTA and Tris-Acetate-EDTA, need to be free from DNAse and RNAse activity. Medicago's SmartBuffers meet all these criteria. They are manufactured using highly purified, analytical grade chemicals and are extensively quality tested. Manufacturing is done in a controlled clean environment according to GMP procedures.
A high level of automation essentially avoids operator intervention and contact with the products.
SmartBuffers are supplied as exactly pre-weighed tablets packed in containers or blister packs or as exactly pre-weighed powder mixes packed in sealed aluminium foil pouches.
•Pre-mixed powder buffers or tablets with pre-set pH
•Analytical grade reagents
•Eliminates calculation, formulation and weighing errors
•Dissolve-and-go for greater convenience
•Stable at room temperature for at least 3 years
•Guaranteed reproducibility
Borate Buffered Saline (BBS)
Borate Buffered Saline (BBS) pH 8.2, 500 ml
Medicago AB
Description
Borate buffered saline (BBS) is commonly used in coating procedures for ELISA and as a component in blocking solutions for Western blotting. The buffer can be used as a sample diluent in biological studies and for various applications within biology and biochemistry to maintain the pH.
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Protocols
Specifications
Chemicals: Analytical grade
Format: Exactly pre-weighed tablets
Composition: 0.15 M NaCl 0.010 M Borate buffer, pH 8.2 at 25°C
Volume: 500 ml
pH: 8.2 ± 0.05 at 25°C
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200
Notes
Specifications
Chemicals: Analytical grade
Format: Exactly pre-weighed tablets
Composition: 0.15 M NaCl 0.010 M Borate buffer, pH 8.2 at 25°C
Volume: 500 ml
pH: 8.2 ± 0.05 at 25°C
Shipping and storage
BBS buffer is shipped at room temperature. Store the tablets in a dry place at room temeprature. Shelf life is three years after production date.
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200
Applications & Benefits
Applications
- Protein-coating on microtiter plates
- Blocking solution in blots together with Casein or BSA
- Studies on DNA/RNA
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200