In Vitro Toxicology: Mutagenicity kits
The Ames II / Ames MPF Microfluctuation Assays are all modifications of the traditional Ames plate incorporation assay.
The Ames II assay uses the S. typhimurium strains TA98 and TAMix (a mixture of 6 strains, each detecting a specific base-pair substitution), whereas the Ames MPF® kits are available with the strains TA98, TA100, TA1535, TA1537, E.coli WP2 uvrA or E.coli WP2 pKM101.
Benefits of Xenometrix Ames Mutagenicity Assays over traditional Ames Procedures
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Easy, compact and high throughput liquid format with 384 well plates.
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Simple detection method with easy "readout" (as compared to the traditional assay)
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Kits include all five types strains cited in OECD 471 (CoA supplied), quality controlled according to the same guideline
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Much less test compound required. (10 mg per strain if tested in triplicates, +/- S9, 6 semi-logarithmic compound dilutions)
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Ames MPF is the preferred system for genotoxic impurities due to its low consumption of compound
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Significantly less consumption of S9
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Less environmental pollution due to significantly less "contaminated" waste
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Less user intervention
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Rat liver S9 with S9-100/1537 Booster Solution
Recommended to reduce possible toxic effects of high S9
concentrations in TA100 and TA1537
Xenometrix AG
Description
Recommended to reduce possible toxic effects of high S9 concentrations in TA100 and TA1537
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