The Ames II / Ames MPF Microfluctuation Assays are all modifications of the traditional Ames plate incorporation assay.
The Ames II assay uses the S. typhimurium strains TA98 and TAMix (a mixture of 6 strains, each detecting a specific base-pair substitution), whereas the Ames MPF® kits are available with the strains TA98, TA100, TA1535, TA1537, E.coli WP2 uvrA or E.coli WP2 pKM101.
Benefits of Xenometrix Ames Mutagenicity Assays over traditional Ames Procedures
Easy, compact and high throughput liquid format with 384 well plates.
Simple detection method with easy "readout" (as compared to the traditional assay)
Kits include all five types strains cited in OECD 471 (CoA supplied), quality controlled according to the same guideline
Much less test compound required. (10 mg per strain if tested in triplicates, +/- S9, 6 semi-logarithmic compound dilutions)
Ames MPF is the preferred system for genotoxic impurities due to its low consumption of compound
Significantly less consumption of S9
Less environmental pollution due to significantly less "contaminated" waste
Less user intervention
S9 Cofactor Kit,
S9 co factor kit contains
S9-Buffer-Salts, 0.9 ml S9-G-6-P, 3.5 ml S9-NADP
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