Cambio - Excellence in Molecular Biology

Lab Reagents

Lab Reagents: Absolute Lectins

Absolute Lectins, perfect for Glycoscience Medicago is a primary manufacturer of a wide variety of exceptionally pure lectins purified by affinity chromatography. Lectins are non-enzymatic proteins of non-immunoglobulin origin that bind specifically and reversibly to carbohydrate moieties without altering the covalent structure of the glycosyl ligands. The term ‘lectin’ is derived from the latin word legere, meaning ‘to select’. The specificity of a lectin is usually defined by the monosaccharides or oligosaccharides that are best at inhibiting the agglutination or precipitation caused by the lectin. Lectins are usually of plant origin but do occur in many types of organism; some are glycoproteins and may be soluble or membrane-bound.

The biological function of lectins is far from fully explored. Their specificity enables binding to glycoproteins and polysaccharides as well as agglutination of erythrocytes and stimulation of blood lymphocytes. Because of their ability to distinguish glycosyl ligands on human red blood cells, lectins can be used for blood typing.Immobilized lectins can be used in affinity chromatography to purify and isolate glycoproteins, glycolipids, polysaccharides, viruses and cells.

Carbohydrate-containing substances bound to the lectin may be eluted with a competitive binding substance. Medicago offers lectins as a freeze-dried powder or as a solution manufactured under aseptic conditions. If you don’t find the lectin you are looking for, we can certainly produce it for you. Furthermore, we offer different custom lectin conjugates on request.

•Ultrapure quality

•High activity

•Wide range

•Lyophilized powder

•Custom lectins and conjugates

Glycine max lectin (SBA)

Glycine max lectin is isolated from soy bean (Soy bean agglutinin, SBA) and purified by affinity chromatography. The molecular weight of the lectin is 120 kDa, it consists of four identical subunits of 30 kDa each and it displays carbohydrate binding specifity for N-acetyl-D-galactosamine and galactopyranosyl residues of glycoproteins

Medicago AB

Catalogue No.DescriptionPack SizePriceQty
  • Change to:
  • £
MC-05-0117-10Glycine max lectin (SBA) 10mg €236.40 Quantity Add to Order
MC-05-0117-50Glycine max lectin (SBA) 50 mg €462.00 Quantity Add to Order

Description

Product description

Glycine max lectin is isolated from soy bean (Soy bean agglutinin, SBA) and purified by affinity chromatography. The molecular weight of the lectin is 120 kDa, it consists of four identical subunits of 30 kDa each and it displays carbohydrate binding specificity for N-acetyl-D-galactosamine and galactopyranosyl residues of glycoproteins (1).

SBA has specificity for blood groups A1, A2 and B. The lectin interacts better with neuramidase-treated cells than with untreated cells. It has selective affinity for lymphocytes and human CD34 hematopoietic stem cells. Immobilized conjugates of SBA are therefore important tools for removing T-cells in bone marrow transplantation.

Medicago’s Glycine max lectin is supplied as a white to cream coloured lyophilized powder from a buffer containing 50 mM NH4HCO3, no preservatives are added. The material is made from a single production batch and is homogenous. It is not derived from any material of animal origin. The lectin is available in vials containing 50 mg or 10 mg lyophilized powder and the product is to be used for laboratory work only.

Features 

  • Ultrapure quality
  • Binding specificity for N-acetyl-D-galactosamine
  • Specificity for blood group: A1 > A2 >> B
  • Lyophilized powder

 

Directions for use

The lectin may be reconstituted with 2 ml of deionized water before use. Spin the vial gently until full dissolution. Aggregation is thought to occur in the presence of high concentrations of 2-mercaptoethanol. The solution may be reconstituted in this buffer to desired working concentration. In absence of lactose the lectin will polymerize and storage at pH 8.6–8.8 causes precipitation.

 

Shipping and storage

The product is shipped at -20°C however for over-the-day transport it may be shipped at ambient temperature. The lyophilized powder is stable for more than three years from production date when stored below -20°C. After reconstitution with deionized water, the solution may be stored frozen in working aliquots for up to 12 months.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

References

References

(1) Liener I. E., Sharon N., Goldstein I. J., (1986) The Lectins – Properties, Functions and Applications in Biology and Medicine.

(2) X-ray crystallographic studies of unique cross-linked lattices between four isomeric biantennary oligosaccharides and soybean agglutinin. Olsen, L.R., Dessen, A., Gupta, D., Sabesan, S., Sacchettini, J.C., Brewer, C.F. (1997) Biochemistry 36: 15073–80.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Notes

Specifications

Appearance: White to cream coloured lyophilized powder                                

Source: Soy bean

Molecular weight: 120 kDa

Sugar specificity: D-GalNAc

Activity: Less than 4 μg/ml will agglutinate fresh A1 cells. Older B cells can be stronger than A2 cells. Blood group specificity: A1 > A2 >> B

Microorganisms: < 100 CFU/g

Protein content: > 80 %, OD280nm (ε 1.28 = 1 mg/mL)

Identity: SDS-PAGE, one band corresponding to the four identical subunits

Shelf life: Three years when stored at -20°

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Applications & Benefits

Applications

  • Studies of SBA-binding normal and tumour cells
  • Blood group agglutination
  • Glycoprotein studies
  • Cell agglutination studies

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200