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Lab Reagents

Lab Reagents: Absolute Lectins

Absolute Lectins, perfect for Glycoscience Medicago is a primary manufacturer of a wide variety of exceptionally pure lectins purified by affinity chromatography. Lectins are non-enzymatic proteins of non-immunoglobulin origin that bind specifically and reversibly to carbohydrate moieties without altering the covalent structure of the glycosyl ligands. The term ‘lectin’ is derived from the latin word legere, meaning ‘to select’. The specificity of a lectin is usually defined by the monosaccharides or oligosaccharides that are best at inhibiting the agglutination or precipitation caused by the lectin. Lectins are usually of plant origin but do occur in many types of organism; some are glycoproteins and may be soluble or membrane-bound.

The biological function of lectins is far from fully explored. Their specificity enables binding to glycoproteins and polysaccharides as well as agglutination of erythrocytes and stimulation of blood lymphocytes. Because of their ability to distinguish glycosyl ligands on human red blood cells, lectins can be used for blood typing.Immobilized lectins can be used in affinity chromatography to purify and isolate glycoproteins, glycolipids, polysaccharides, viruses and cells.

Carbohydrate-containing substances bound to the lectin may be eluted with a competitive binding substance. Medicago offers lectins as a freeze-dried powder or as a solution manufactured under aseptic conditions. If you don’t find the lectin you are looking for, we can certainly produce it for you. Furthermore, we offer different custom lectin conjugates on request.

•Ultrapure quality

•High activity

•Wide range

•Lyophilized powder

•Custom lectins and conjugates

Phaseolus vulgaris lectin L (PHA-L)

Phaseolus vulgaris lectin L (PHA-L) is isolated from red kidney beans and purified by affinity chromatography. It is a tetrameric protein with a molecular weight of 126 kDa, each subunit of about 31 kDa. The lectin recognizes and binds specifically to terminal galactose, N-acetylglucosamine and mannose residues of complex glycans on mammalian glycoproteins

Medicago AB

Catalogue No.DescriptionPack SizePriceQty
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MC-05-0132-10Phaseolus vulgaris lectin L (PHA-L) 10 mg €388.80 Quantity Add to Order
MC-05-0132-2Phaseolus vulgaris lectin L (PHA-L) 2 mg €192.00 Quantity Add to Order

Description

Product description

Phaseolus vulgaris lectin L (PHA-L) is isolated from red kidney beans and purified by affinity chromatography. It is a tetrameric protein with a molecular weight of 126 kDa, each subunit of about 31 kDa. The lectin recognizes and binds specifically to terminal galactose, N-acetylglucosamine and mannose residues of complex glycans on mammalian glycoproteins (1).

PHA-L is also known as leucoagglutinin and has high mitogenic and leucoagglutinating activity, but low erythroagglutinating activity (1). The lectin recognizes terminal galactose residues of complex glycans on mammalian glycoproteins such as thyroglobulin (3). PHA-L does not agglutinate human erythrocytes at concentrations of 250 µg/ml or less, and is non-specific for blood groups.

Medicago’s PHA-L lectin is supplied as a white lyophilized powder from a buffer containing from 10 mM NH4HCO3. The purity of the lectin is determined by SDS-PAGE generating one single band at 31 kDa. It is available in vials containing 10 mg or 2 mg lyophilized powder and the product is to be used for laboratory work only.


Features 

  • Ultrapure quality
  • Sugar specificity: complex oligosaccharides
  • High mitogenic and leucoagglutinating activity
  • Very low erythroagglutinating activity
  • Lyophilized powder

 

 

Directions for use

The lectin may be reconstituted with 2 ml of deionized water before use. Spin the vial gently until full dissolution. Aggregation is thought to occur in the presence of high concentrations of 2-mercaptoethanol. The solution may be reconstituted in this buffer to desired working concentration.

 

Shipping and storage

The product is shipped at -20°C however for over-the-day transport it may be shipped at ambient temperature. The lyophilized powder is stable for more than three years from production date when stored below -20°C. After reconstitution with deionized water, the solution may be stored frozen in working aliquots for up to 12 months.

 

 

 

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References

References 

(1) Liener I. E., Sharon N., Goldstein I. J., (1986) The Lectins – Properties, Functions and Applications in Biology and Medicine.

(2) Weak protein-protein interactions in lectins: the crystal structure of a vegetative lectin from the legume Dolichos biflorus. Buts, L., Dao-Thi, M.H., Loris, R., Wyns, L., Etzler, M., Hamelryck, T. (2001) J.Mol.Biol. 309: 193–201.

(3) Chrispeels M. J., Raikhel N. V. (1991). Lectins, Lectin Genes, and Their Role in Plant Defense. The Plant Cell Vol.3, 1–9.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Notes

Specifications

Appearance: White lyophilized powder or flocculate

Source: Red kidney beans

Molecular weight: 126 kDa

Sugar specificity: D-GalNAc

Activity: No agglutination at 250 μg/ml of a 2% suspension of human erythrocytes after 1 h at 25°C.                         

Microorganisms: < 100 CFU/g

Protein content: > 85 %, OD280

Identity: SDS-PAGE, one band at 31 kDa

Shelf life: > Three years when stored at -20°

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Applications & Benefits

Applications

  • Leucocyte agglutination studies
  • Mammalian glycoprotein studies
  • Model system of how proteins recognize carbohydrates

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200