Vicia ervilia lectin or agglutinin (VEA) is isolated from bitter vetch seeds and has a molecular weight of 53 kDa. This lectin is blood group non-specific with sugar specificity for α-mannose and, to a lesser extent, α-glucose residues (1). Agglutination is inhibited by mannose, glucose, fructose, methyl mannoside, maltose, melezitose and, α, α´-trehalose (1).
Medicago’s VEA lectin is supplied as a yellow lyophilized powder without preservatives. The purity of VGA is determined by SDS- electrophoresis. The lectin is available in vials containing 50 mg or 10 mg powder and the product is to be used for laboratory work only.
Directions for use
The lectin may be reconstituted with 2 ml of deionized water before use. Spin the vial gently until full dissolution. Aggregation is thought to occur in the presence of high concentrations of 2-mercaptoethanol.
Shipping and storage
The product is shipped at -20°C however for over-the-day transport it may be shipped at ambient temperature. The lyophilized powder is stable for more than three years from production date when stored below -20°C. After reconstitution with deionized water, the solution may be stored frozen in working aliquots for up to 12 months.
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200