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Oligo Synthesis

Oligo Synthesis : Click Chemistry

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C8-Alkyne-dC-CE Phosphoramidite

C8-Alkyne-dC-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1543-02C8-Alkyne-dC-CE Phosphoramidite0.25g £1,136.00 Quantity Add to Order
10-1543-90C8-Alkyne-dC-CE Phosphoramidite100umole £432.00 Quantity Add to Order
10-1543-95C8-Alkyne-dC-CE Phosphoramidite50umole £227.00 Quantity Add to Order

Description

Structure

Catalog Number: 10-1543-xx

Description: C8-Alkyne-dC-CE Phosphoramidite

5'-Dimethoxytrityl-N4-benzoyl-5-(octa-1,7-diynyl)-2'-deoxycytidine,
3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C54H60N5O8P M.W.: 938.06 F.W.: 393.33
Diluent: Anhydrous Acetonitrile
Coupling: 3 minute coupling time recommended.
Deprotection: Ammonium hydroxide for 2 hours at 55 °C or as required by nucleobases.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 1-2 days

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Protocols

Glen Report 23.1: New Products - Click Chemistry Update

Material Safety Data Sheet

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References

1. The Glen Report, 2010, 22, 1-4.

2. J. Gierlich, G.A. Burley, P.M. Gramlich, D.M. Hammond, and T. Carell, Org Lett,2006, 8, 3639-42.

3. F. Seela, and V.R. Sirivolu, Chem Biodivers, 2006, 3, 509-14.

4. P.M.E. Gramlich, S. Warncke, J. Gierlich, and T. Carell, Angewandte Chemie International Edition, 2008, 47, 3442-3444.

5. P.M.E. Gramlich, C.T. Wirges, A. Manetto, and T. Carell, Angewandte Chemie International Edition, 2008, 47, 8350-8358.

6. K. Li, L.A. Lee, X. Lu, and Q. Wang, BioTechniques, 2010, 49, 525-7.

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Applications & Benefits

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1543-02 0.25grams .25grams 2.67 75.67 45.4 28.38 20.64 15.13 3.78
10-1543-90 100µmoles .094grams 1 20 12 7.5 5.45 4 1
10-1543-95 50µmoles .047grams .5 3.33 2 1.25 .91 .67 .17
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1543-02 0.25grams .25grams 3.98 .07 73.2 45.75 33.27 4.58
10-1543-90 100µmoles .094grams 1.5 .07 23.6 14.75 10.73 1.48
10-1543-95 50µmoles .047grams .75 .07 8.6 5.38 3.91 .54
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1543-02 0.25grams .25grams 3.98 .07 74.8 46.75 34

10-1543-90 100µmoles .094grams 1.5 .07 25.2 15.75 11.45

10-1543-95 50µmoles .047grams .75 .07 10.2 6.38 4.64

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Related products

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