Cambio - Excellence in Molecular Biology

Cell Culture Contamination Control

Cell Culture Contamination Control: Detection

Mycoplasma Detection

As an efficient contamination control measure we recommend frequent routine testing of cell culture materials with Venor®Gem Mycoplasma Detection Kits. These PCR- and qPCR-based mycoplasma assays allow fast (within a few hours), simple, effective and sensitive detection of mycoplasma-contaminated cell cultures in any standard molecular biology laboratory.

Regular mycoplasma testing and control procedures can significantly help in avoiding the costly consequences of contamination ensuring safe biopharmaceuticals and reliable scientific results.  Many scientific journals currently do not accept submissions of cell-based studies without evidence that mycoplasma testing has been performed.

To meet different customer needs Minerva Biolabs has developed a range of Venor®GeM mycoplasma detection assays using conventional PCR or qPCR methods which enable rapid and reliable identification of mycoplasma in cell cultures, culture supernatants and tissue culture media.

Conventional PCR

  • Venor®GeM Classic allows fast, reliable and time-saving routine monitoring of mycoplasma contamination in cell culture supernatants, media and biopharmaceuticals in research and industry by conventional PCR.

  • Venor®GeM OneStep is a kit for direct detection. It requires minimal pipetting, the kit includes all reagents required for PCR in a ready-to-use lyophilized reaction mix. Only samples or controls need to be added prior to PCR.

  • Venor®GeM Advance is designed for direct detection. Designed to reduce the total assay time and the pipetting steps, the kit contains PCR strips of tubes, each pre-dispensed with all reagents necessary for each PCR reaction, including the polymerase. For additional convenience, the gel loading buffer and dye are already included in the reaction buffer. After PCR, the products can be loaded directly on an agarose gel.

Real-time PCR (qPCR)

Our qPCR kits are TaqMan®-based qPCR Assays with labelled probes:  FAM® channel (mycoplasma) and HEX® channel (internal control to check for PCR inhibition from contaminants in sample).  So not only does the qPCR approach remove a lot of hassle, but the internal controls also give you reassurance that your negatives are not false.

  • Venor®GeM qEP utilises quantitative, real-time PCR (qPCR) for fast (approx. 3 h) and reliable screening of cell culture supernatants for mycoplasma contamination. The kit can be used in combination with any type of real-time PCR cycler able to detect the fluorescence dyes FAM® and HEX®. For clinical diagnostics in which human samples are tested we recommend using this kit as it conforms to EP 2.6.7, USP <71> and JP G3. It is suitable for both research and industry where certified compliance is needed or desirable. 

  • Venor®GeM qOneStep is a kit based on a real-time PCR (qPCR) assay for rapid, robust and sensitive detection of mycoplasma contamination. The detection procedure can be performed within 3 hours. Primers, nucleotides, probes, polymerase and an internal amplification control are provided as a ready-to-use, lyophilized reaction mix. This kit is a very straightforward and is highly suitable for academic labs and industrial applications where certified compliance of kits is not required.


Venor®GeM OneStep for conventional, endpoint PCR

Venor®GeM OneStep is a complete kit which includes all reagents required for PCR. Primer, nucleotides, polymerase and the internal amplification control are provided ready-to-use in a lyophilized reaction mix. The included rehydration buffer is added to the mix, aliquots made according to the sample number, sample or Positive Control DNA added and the setup is ready for PCR.

Minerva Biolabs

Catalogue No.DescriptionPack SizePriceQty
11-8025Venor®GeM OneStep25 tests £156.00 Quantity Add to Order
11-8050Venor®GeM OneStep50 tests £286.00 Quantity Add to Order
11-8100Venor®GeM OneStep100 tests £502.00 Quantity Add to Order
11-8250Venor®GeM OneStep250 tests £1,067.00 Quantity Add to Order

Description

Venor®GeM OneStep


Venor®GeM OneStep is a complete kit which includes all reagents required for PCR. Primer, nucleotides, polymerase and the internal amplification control are provided ready-to-use in a lyophilized reaction mix. The included rehydration buffer is added to the mix, aliquots made according to the sample number, sample or Positive Control DNA added and the setup is ready for PCR.


Type of PCR
For conventional, endpoint PCR


Scope of Application
Applicable in research and industry for direct testing of cell cultures and biologicals. Intended for research use only. Not recommended for clinical diagnostics or testing of human samples.

Kit Components
Primer sets, nucleotides, internal amplification control and polymerase at optimized concentrations lyophilized in one tube
Rehydration buffer
Lyophilized positive control
Tris/EDTA buffer
Package Sizes
Primer sets and nucleotides are prepared in aliquots of 25 tests.

    Cat. No. 11-8025 25 Tests
    Cat. No. 11-8050 50 Tests
    Cat. No. 11-8100 100 Tests
    Cat. No. 11-8250 250 Tests

Result evaluation

Gel electrophoresis at endpoint of PCR
Required Consumables
Agarose gels and DNA stains
PCR reaction tubes
Required lab devices
Gel electrophoresis and documentation system / Regular PCR cycler
Pipetting equipment
Tube centrifuge
Shelf Life and Storage
Venor®GeM OneStep components are maintainable at 2 to 8 °C for at least 6 months. After rehydratisation the reagents must be stored at -18 °C.

EP 2.6.7 compliance
No

 

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Protocols

Venor®GeM OneStep protocol

Please note: all protocols off site are the responsibility of the products supplier

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

References

For a list of references please click here

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Applications & Benefits

Applicable in research and industry for direct testing of cell cultures and biologicals. Intended for research use only. Not recommended for clinical diagnostics or testing of human samples.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200