Epicentre's® NTPs are provided as sterile, neutral solutions of ATP, CTP, GTP, or UTP, each at 10mM, or as a premixed solution of all four NTPs at 2.5mM each for use in RNA synthesis.
The ATP Solution is also suitable for use with T4 DNA Ligase for ligating DNA molecules, with T4 RNA Ligase for ligating RNA molecules, with T4 Polynucleotide Kinase for phosphorylating the 5'-ends of DNA or RNA, or with Plasmid-Safe™ ATP-Dependent DNase for digesting linear DNA.
Epicentre's® NTPs are provided as sterile, neutral solutions of ATP, CTP, GTP, or UTP, each at 10mM, or as a premixed solution of all four NTPs at 2.5mM each for use in RNA synthesis.
The ATP Solution is also suitable for use with T4 DNA Ligase for ligating DNA molecules, with T4 RNA Ligase for ligating RNA molecules, with T4 Polynucleotide Kinase for phosphorylating the 5'-ends of DNA or RNA, or with Plasmid-Safe™ ATP-Dependent DNase for digesting linear DNA.
Storage Buffer
NTP Solutions are made with sterile deionised water and adjusted to pH 7.0.
ATP (Adenosine-5'-Triphosphate)
Formula (free acid): C10H16N5O13P3
Formula weight: 507
A250/A260 = 0.80
A280/A260 = 0.14
CTP (Cytidine-5'-Triphosphate)
Formula (free acid): C9H16N3O14P3
Formula weight: 483
A250/A260 = 0.44
A280/A260 = 2.03
GTP (Guanosine-5'-Triphosphate)
Formula (free acid): C10H16N5O14P3
Formula weight: 523
A250/A260 = 1.14
A280/A260 = 0.65
UTP (Uridine-5'-Triphosphate)
Formula (free acid): C9H15N2O15P3
Formula weight: 484
A250/A260 = 0.74
A280/A260 = 0.36
2'-Fluoro-dUTP (2'-F-dUTP)
Formula: C9H10N2O14FP3Li4
MW: 509.86
Purity: >90%
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2'-Fluoro-dCTP (2'-F-dCTP)
Formula: C9H11N3O13FP3Li4
MW: 508.88
Purity: >90%
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