The GST tag is a 26 kDa protein (glutathione-S-transferase) that binds to glutathione-coupled agarose resins. This tag is considerably larger than the typically 6x or 10x His-tags and is often chosen to promote recombinant protein folding. Cambio Glutathione Agarose is produced using BioWorks Workbeads that have exceptional chromatography characteristics.
For purification from cell culture supernatants or pull-down experiments, we recommend Cambio Glutathione MagBeads.
Cambio Glutathione Agarose provides:
- protein binding capacity of up to 10 mg/mL
- compatible with a range of detergents and additives
- ideal for purifying insoluble proteins
Cambio Glutathione Agarose has been shown to purify GST-tagged proteins with comparable yields and purity to leading brands available from other suppliers.
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200