Cambio - Excellence in Molecular Biology

In vitro Transcription

In vitro Transcription: In Vitro Transcription

ScriptCap™ m7G Capping System

For post-transcriptional capping of in vitro transcribed RNA with ScriptCap Capping Enzyme, GTP and SAM. Cap 0 (N7-methyl-G) caps can be built with efficiencies approaching 100%. This cannot be accomplished with co-transcriptional capping methods. Use in conjunction with ScriptCap 2'-O-Methyltransferase to produce Cap 1 caps, that which is found on most eukaryotic mRNAs.

CellScript

Catalogue No.DescriptionPack SizePriceQty
C-SCCE0625ScriptCap™ m7G Capping System25 Reactions £323.00 Quantity Add to Order

ScriptCap™ m7G Capping System

For post-transcriptional capping of in vitro transcribed RNA with ScriptCap Capping Enzyme, GTP and SAM. Cap 0 (N7-methyl-G) caps can be built with efficiencies approaching 100%. This cannot be accomplished with co-transcriptional capping methods. Use in conjunction with ScriptCap 2'-O-Methyltransferase to produce Cap 1 caps, that which is found on most eukaryotic mRNAs.

CellScript

ScriptCap™ m7G Capping System catalyzes in vitro addition of a cap nucleotide to the 5' terminus of primary RNA that has a 5'-triphosphate group, such as RNA obtained from an in vitro transcription reaction. A "cap nucleotide" or “cap” is a guanine nucleoside that is joined via its 5' carbon to a triphosphate group that is, in turn, joined to the 5' carbon of the most 5' nucleotide of the primary mRNA transcript, and in most eukaryotes, the nitrogen at the 7 position of guanine in the cap nucleotide is methylated. Such a capped transcript can be represented as:

 m7G(5')ppp(5')N1(pN)x-OH(3')

where m7G represents the 7-methylguanosine cap nucleoside, ppp represents the triphosphate bridge between the 5' carbons of the cap nucleoside and the first nucleotide of the primary RNA transcript, and N1(pN)x-OH(3') represents the primary RNA transcript, of which N1 is the most 5' nucleotide.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

ScriptCap™ m7G Capping System

For post-transcriptional capping of in vitro transcribed RNA with ScriptCap Capping Enzyme, GTP and SAM. Cap 0 (N7-methyl-G) caps can be built with efficiencies approaching 100%. This cannot be accomplished with co-transcriptional capping methods. Use in conjunction with ScriptCap 2'-O-Methyltransferase to produce Cap 1 caps, that which is found on most eukaryotic mRNAs.

CellScript

Protocols for: ScriptCap™ m7G Capping System

ScriptCap™ m7G Capping System Protocol 

(catalogue number SCCE610 / SCCE625) 

Please note: all protocols are the responsibility of the product supplier

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

ScriptCap™ m7G Capping System

For post-transcriptional capping of in vitro transcribed RNA with ScriptCap Capping Enzyme, GTP and SAM. Cap 0 (N7-methyl-G) caps can be built with efficiencies approaching 100%. This cannot be accomplished with co-transcriptional capping methods. Use in conjunction with ScriptCap 2'-O-Methyltransferase to produce Cap 1 caps, that which is found on most eukaryotic mRNAs.

CellScript

1.Kuge, H. et al., (1998) Nucl. Acids Res. 26, 3208.

2.Konarska, M.M. et al., (1984) Cell 38, 731.

3.Jemielity, J. et al., (2003) RNA 9, 1108.

4.Grudzien, E. et al., (2004) RNA 10, 1479.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

ScriptCap™ m7G Capping System

For post-transcriptional capping of in vitro transcribed RNA with ScriptCap Capping Enzyme, GTP and SAM. Cap 0 (N7-methyl-G) caps can be built with efficiencies approaching 100%. This cannot be accomplished with co-transcriptional capping methods. Use in conjunction with ScriptCap 2'-O-Methyltransferase to produce Cap 1 caps, that which is found on most eukaryotic mRNAs.

CellScript

 

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

ScriptCap™ m7G Capping System

For post-transcriptional capping of in vitro transcribed RNA with ScriptCap Capping Enzyme, GTP and SAM. Cap 0 (N7-methyl-G) caps can be built with efficiencies approaching 100%. This cannot be accomplished with co-transcriptional capping methods. Use in conjunction with ScriptCap 2'-O-Methyltransferase to produce Cap 1 caps, that which is found on most eukaryotic mRNAs.

CellScript

Benefits 

    • 100% capped RNA. 
    • Cap 1 structure.  (if used in conjunction with ScriptCap™ 2'-O-Methyltransferase),
    • Proper cap orientation.
    • High yields.
    • Low cost

 

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200