Cell Line Information:
Organism: Rattus norvegicus (rat)
Tissue: pheochromocytoma; adrenal gland
Morphology: small irregularly shaped cells
Tumorigenic: yes, in New England Deaconess Hospital strain rats
Karyotype: 40 chromosomes; 38 autosomes plus XY
Products: catecholamines; dopamine; norepinephrine
Receptors expressed: nerve growth factor (NGF)
Growth Properties: floating clusters; few scattered lightly attached cells.
Depositor: B. Patterson
Comments: The PC-12 cell line was derived from a transplantable rat pheochromocytoma. The cells respond reversibly to NGF by induction of the neuronal phenotype when plated on Collagen IV coated culture flasks. The cells do not synthesize epinephrine.
The Transfection Reagent:
Transfection of suspension cells, or suspension/adherent mixed cells, such as PC-12 Cells, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.
PC-12 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting PC-12 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for PC-12 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
- Specifically optimized to deliver nucleic acids into PC-12 Cells
- Highest efficiency to ensure experimental success
- Extremely gentle to cells
- Deliver single or multiple plasmids
- 100% animal-free
- Simplest and straightforward protocols
- Compatible with serum
- Suitable for Reverse Transfection
- Compatible with transfection in any plate formats
- Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
|FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on PC-12 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for PC-12 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this PC-12 Cell Avalanche Transfection Reagent.
For Other Cells:
|PC-12 Cell Avalanche™ Transfection Reagent (rat pheochromocytoma cell) can also be used on the following cells with high transfection efficiencies.
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200