Cell Line Information:
Designations: Ramos (RA 1)
Depositors: BC Giovanella
Isotype: IgM; lambda light chain
Growth Properties: suspension
Organism: Homo sapiens
Source: Burkitt's lymphoma (American)
Cell Type: B lymphocyte;
Cellular Products: immunoglobulin (surface and secreted)  .
Applications: transfection host
Receptors: interleukin 4 (IL-4); low affinity IgE (CD23) 
Antigen Expression: CD23 
Age: 3 years
Comments: The cells are negative for Epstein-Barr virus. They have about 1500 IL-4 binding sites per cell.
The cells are reported to secrete IgM (lambda light chain).
The Transfection Reagent:
Transfection of suspension cells, such as Ramos Cells, has always been challenging, both technically and financially. The most common systems currently used for relatively efficient molecular delivery to these cells are expensive electroporation instruments.
Ramos Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on Ramos Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Ramos Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of Ramos Cell Avalanche™ Transfection Reagent for transfection on Ramos Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
- Specifically optimized to deliver nucleic acids into Ramos Cells
- Highest efficiency to ensure experimental success
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- Simplest and straightforward protocols
- Compatible with serum
- Suitable for Reverse Transfection
- Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
|FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on RAMOS cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for RAMOS cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this RAMOS Cell Avalanche Transfection Reagent.
For Other Cells:
|Ramos Cell Avalanche™ Transfection Reagent (human lymphoma cell) can also be used on the following cells with high transfection efficiencies.
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
DU 145 Cell
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