The highly versatile TransforMax™ EC100™ E. coli competent cells are ideal for most cloning applications. The cells provide very high transformation efficiency when tested against a wide range of supercoiled DNAs as well as DNA directly from a ligation reaction (Table 1).

Table 1. Comparison of the transformation efficiencies of TransforMax™ EC100™ E. coli with a variety of DNAs. Transformations were performed using 50 µl of competent cells and either supercoiled DNAs of the indicated sizes or a 1-µl aliquot from a standard 10-µl ligation reaction. Results shown are in cfu/µg of DNA and are the average transformation efficiencies obtained from several trials.

Genotype

F^- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ^- rpsL (Str^R) nupG

TransforMax EC100 Electrocompetent E. coli

• Transformation efficiency of >1 x 10¹⁰ cfu/µg of pUC19.

TransforMax EC100 Chemically Competent E. coli

• Transformation efficiency of >5 x 10⁸ cfu/µg of pUC19.

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Protocols for: TransforMax™ EC100™ Electrocompetent E. coli & TransforMax™ EC100™ Chemically competent E. coli

TransforMax™ EC100™ Electrocompetent E. coli Protocol

(catalogue number EC10010)

TransforMax™ EC100™ Chemically competent E. coli Protocol

(catalogue number CC02810)

Please note: all protocols off site are the responsibility of the product supplier

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Citations for TransforMax™ EC100™ Electrocompetent E. coli and
TransforMax™ EC100™ Chemically Competent E. coli

1. Uhlich, G. A. (2009) KatP contributes to OxyR-regulated hydrogen peroxide resistance in Escherichia coli serotype O157 : H7, Microbiology 155 , 3589-3598.
2. Uhlich, G. A., et al. (2009) The CsgA and Lpp Proteins of an Escherichia coli O157:H7 Strain Affect HEp-2 Cell Invasion, Motility, and Biofilm Formation, Infect. Immun. 77 , 1543-1552.
3. Vaezeslami, S., et al. (2007) Site-Directed Mutagenesis Studies of Tn5 Transposase Residues Involved in Synaptic Complex Formation, J. Bacteriol. 189 , 7436-7441.
4. Kloosterman, W. P., et al. (2006) Cloning and expression of new microRNAs from zebrafish, Nucleic Acids Res. 34 , 2558-2569.
5. Sanseverino, J., et al. (2005) Use of Saccharomyces cerevisiae BLYES Expressing Bacterial Bioluminescence for Rapid, Sensitive Detection of Estrogenic Compounds, Appl. Envir. Microbiol. 71 , 4455-4460.
6. Dorsey, C. W., et al. (2004) The siderophore-mediated iron acquisition systems of Acinetobacter baumannii ATCC 19606 and Vibrio anguillarum 775 are structurally and functionally related, Microbiology 150 , 3657-3667.
7. Wyborn, N. R., et al. (2004) Properties of haemolysin E (HlyE) from a pathogenic Escherichia coli avian isolate and studies of HlyE export, Microbiology 150 , 1495-1505.

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Applications

• Great for general cloning applications where high transformation efficiencies are desired
• Routine cloning of DNA up to 200 kb.

Benefits

• High transformation efficiency with clones of all sizes, including BAC clones (Table 1).
• lacZΔM15 for blue/white screening of recombinants.
• Restriction minus [mcrA, Δ(mrr-hsdRMS-mcrBC)] enables efficient cloning of methylated DNA.
• Endonuclease minus (endA1) to ensure high yields of DNA.
• Recombination minus (recA1) for greater stability of large cloned inserts

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200