Cell Line Information:
Cell Line Name: SK-MEL-5 (ATCC® HTB-70™)
Organism: Homo sapiens, human
Tissue: skin, derived from metastatic axillary node.
Cell Type: Melanoma
Culture Properties: adherent
Biosafety Level: 1
Disease: malignant melanoma
Age: 24 years
Markers: Blood Type O; Rh ; HLA A2, A11, B40, Bw16
Comments: This is one of a very extensive series of melanoma lines (see also ATCC HTB-71, ATCC HTB-72 and ATCC HTB-73) isolated by T. Takahashi and associates. The lines served as source of target cells for detection of melanoma specific antibody in patients with the disease.
Institution: The Memorial Sloan-Kettering Cancer Center
SK-MEL-5 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.
The Transfection Reagent:
SK-MEL-5 Cell Avalanche® Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting SK-MEL-5 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for SK-MEL-5 cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?
- Unique formulation-maximize transfection performance in SK-MEL-5 cells
- Extremely gentle to cells
- Deliver single or multiple plasmids
- Free of animal-derived components
- Simplest and straightforward protocols to Increase experimental throughput
- Active in serum-containing medium
- Suitable for Reverse Transfection
- Compatible with transfection in any plate format
- Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
- Great pricing
- Developed and manufactured by EZ Biosystems
|FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on SK-MEL-5 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for SK-MEL-5 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this SK-MEL-5 Cell Avalanche® Transfection Reagent.
For Other Cells:
|SK-MEL-5 Cell Avalanche® Transfection Reagent (human melanoma cell) can also be used on the following cells with high transfection efficiencies.
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200