Cambio - Excellence in Molecular Biology

Extraction & Purification - DNA

Extraction & Purification - DNA: Clinical

Isolate and purify DNA from clinical materials, such as buccal swabs, blood, saliva tissues and stool samples.

Exgene™ Cell SV

Exgene™ Cell SV kit provides a rapid and simple method for the purification of total DNA from a wide range of organism including bacterial cells, yeast, cultured cells, whole blood and blood derivatives. Up to 2 x 109 bacterial cells, 5 x 106 cultured cells or 3 ml of yeast cultures may yield 10 ~ 40 ? of DNA typically.

GeneAll

Description

Description

ExgeneTM Cell SV kit provides a rapid and simple method for the purification of total DNA from a wide range of organisms including bacterial cells, yeast, cultured cells, whole blood and blood derivatives.
Up to 2 x 109 bacterial cells, 5 x 106 cultured cells or 3 ml of yeast cultures may yield between 10 ug to ~ 40ug of DNA typically. The pure DNA can be acquired in just 30 minutes and this can be directly used in various applications such as PCR, Southern blotting and other enzymatic reactions.

 

Features and Benefits

¦ Spin or vacuum column format
¦ Accurate and consistent DNA extraction from gram positive or negative bacteria, cultured cell, yeast and
   various biological samples
¦ High purity : 1.8 ~ 2.0
¦ Simple and safe procedure
¦ No use of organic solvents
¦ Ready for use in PCR, Southern blotting, AFLP, RFLP, RAPD and other enzymatic reactions

Click here for protocol ( Maxi kit)

Click here for Protocol ( Mini kit)

Click here to see the range of sample types that
the ExgeneTM Cell SV kit has been sucessfull with.
 

 

 

 

 

Technical Information

Features Cell SV mini Cell SV MAXI
Format Column type G (mini) with collection tubeColumn type G (MAXI) with collection tube
Maximum sample size
 ~2 x 109 bacterial cells
~5 x 107 yeast cells
~5 x 1010 bacterial cells
~5 x 108 yeast cells
Preparation time30~120 minutes

60~240 minutes 

Typical yield5~25 μg80~400 μg
Elution volume30~400 μl0.4~2 ml
 

Technical Data

 

  Consistent Result from Various Samples

       

Compatibility Test with Restriction Enzymes

       

 

PCR Amplification Test

       

 

 

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Protocols

Click here for protocol ( Maxi kit)

Click here for Protocol ( Mini kit)

 

Click here for flyer 

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

References

List of references here

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Notes

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Related products

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200