Description
ExgeneTM Cell SV kit provides a rapid and simple method for the purification of total DNA from a wide range of organisms including bacterial cells, yeast, cultured cells, whole blood and blood derivatives.
Up to 2 x 109 bacterial cells, 5 x 106 cultured cells or 3 ml of yeast cultures may yield between 10 ug to ~ 40ug of DNA typically. The pure DNA can be acquired in just 30 minutes and this can be directly used in various applications such as PCR, Southern blotting and other enzymatic reactions.
Features and Benefits
¦ Spin or vacuum column format
¦ Accurate and consistent DNA extraction from gram positive or negative bacteria, cultured cell, yeast and
various biological samples
¦ High purity : 1.8 ~ 2.0
¦ Simple and safe procedure
¦ No use of organic solvents
¦ Ready for use in PCR, Southern blotting, AFLP, RFLP, RAPD and other enzymatic reactions
Click here for protocol ( Maxi kit)
Click here for Protocol ( Mini kit)
Click here to see the range of sample types thatthe ExgeneTM Cell SV kit has been sucessfull with.
Technical Information
| Features |
Cell SV mini |
Cell SV MAXI |
| Format |
Column type G (mini) with collection tube | Column type G (MAXI) with collection tube |
| Maximum sample size | ~2 x 109 bacterial cells ~5 x 107 yeast cells | ~5 x 1010 bacterial cells
~5 x 108 yeast cells
|
| Preparation time | 30~120 minutes | 60~240 minutes |
| Typical yield | 5~25 μg | 80~400 μg |
| Elution volume | 30~400 μl | 0.4~2 ml |
Technical Data
Consistent Result from Various Samples
Compatibility Test with Restriction Enzymes
PCR Amplification Test
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200