Telomeric PRINS Kit
Star*FISH© PRINS (PRimed IN situ Synthesis) is a rapid and sensitive method of detecting sequences of DNA without requiring lengthy in situ hybridisation protocols. The technique is basically a single-cycle PCR reaction, requiring a single specific primer and labelled dUTP/dNTP solution.
The key advantages of this method of in situ detection are the intensity and clarity of the signal and the speed of the protocol. The signal produced is intense and localised due to the fact that individual unincorporated nucleotides do not contribute significantly to background, and carefully chosen primers eliminate false signal emerging from other loci. With its speedy protocol, results can be obtained in under 1 hour (a lot faster than conventional FISH). Also, PCR requires few, if any, toxic reagents, unlike the need for formamide in FISH.
To start with, we are only offering a telomere-specific primer, and other probes for other regions of the genome will be developed in due course. In order to keep the price down, and maximise the flexibility of this product, we are not including labelled dUTP or Taq DNA polymerase (although we can supply a licenced Taq on request). Customers can therefore choose labelled dUTP and Taq to suit their experiment, budget and local licensing constraints.
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200