Description
RibospinTM provides a convenient method for isolation of total RNA from cell and tissue samples. RibospinTM procedures employed the glassfibre membrane technology for the fastest and the most convenient of high purity RNA isolation, instead of conventional alcohol precipitation or phenol / chloroform extraction. Whole procedure takes only 15 minutes and the eluates are suitable for RT-PCR or any downstream application without further manipulation.
Features and Benefits
Glassfibre membrane technology
Sample size : Up to 25 mg tissue or up to 5 x 106 cultured cells
Typical yield : Up to 20 ug/ 1 x 106 cultured cells
Up to 60 ug / 10 mg liver tissue
High purity : A260/A230 > 2.0, A260/A280 > 1.8
Preparation time : ~ 15 minutes
Stable and consistent yield
No phenol / chloroform extraction
No ethanol precipitation
Ready for use in RT-PCR, northern blotting, dot blotting, in vitro translation, molecular cloning,
Real-time PCR, RNase protection assays and other analytical procedures
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