Transfection-EZ Biosystems: Cell Lines
Avalanche®- Cell Line-specific Transfection Reagent series:
Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines). Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.
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AR42J Cell Avalanche™ Transfection Reagent (rat pancreas tumor cell)
AR42J Cell Avalanche™ Transfection Reagent (rat pancreas tumor cell)
EZ Biosystems
Description
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Cell Line Information:
Designations: AR42J
Organism: Rattus norvegicus (rat)
Strain: Wistar
Tissue: pancreas; exocrine; tumor
Morphology: epithelial
Growth properties: adherent
Tumorigenic: yes, in athymic mice
Products: amylase and other exocrine enzymes
Receptors expressed: insulin; glucocorticoid
Depositors: N.W. Jessop
The cells grow slowly, in clusters. They tend to pile up and appear refractile. Secretory activity is inducible by glucocorticoid stimulation, and is accompanied by extensive re-organization of the endoplasmic reticulum.
The Transfection Reagent:
AR42J Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on AR42J Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for AR42J Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of AR42J Cell Avalanche™ Transfection Reagent for transfection on AR42J Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
Features:
- Specifically optimized to deliver nucleic acids into AR42J Cells
- Highest efficiency to ensure experimental success
- Extremely gentle to cells
- 0.5 ml is able to transfect about 1000 wells of 24-well plate
- Deliver single or multiple plasmids
- Compatible with serum
- Suitable for Reverse Transfection
- Compatible with transfection in any plate formats
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| Data: |
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| FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on AR42J cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for AR42J cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this AR42J Cell Avalanche Transfection Reagent. |
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| For Other Cells: |
| AR42J Cell Avalanche™ Transfection Reagent (rat pancreas tumor cell) can also be used on the following cells with high transfection efficiencies.
Capan-1 Cell PANC-1 Cell BxPC-3 Cell AsPC-1 Cell 293 Cell 293T/17 Cell SK-OV-3 Cell U-2 OS cell Saos-2 Cell SK-N-SH Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. |
| Research Use Only. Not for use in diagnostic or therapeutic procedures. |
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200
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If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200