T7-FlashScribe™ Transcription Kit V2
- Rapid, high yield production of up to 180 µg of uridine-containing IVT RNA (U-RNA) from 1 µg of template in just 30 minutes.
- Produce high yields of either long or short transcripts.
- Easily scale up reactions to produce milligram amounts of U-RNA.
- ScriptGuard™ RNase Inhibitor included to protect synthesized RNA from RNase degradation.
Product is for research use only (RUO)
Product Description:
The T7-FlashScribe™ Transcription Kit V2 is specially formulated to
maximize yields of canonical uridine-containing RNA (U-RNA) from an in vitro
transcription (IVT) reaction. Although yield varies with the DNA
template and other factors, a standard 30 minute, 20 μl reaction will
yield up to 180 μg of RNA from 1 μg of DNA template. These high yields
are due to the superior performance properties of the T7-FlashScribe™
enzyme. The standard reaction can be scaled up to generate milligram
amounts of either long or short RNA transcripts. ScriptGuard™ RNase Inhibitor is included in the kit to protect synthesized RNA from RNase degradation.
T7-FlashScribe™ IVT U-RNA can be further processed into U-mRNA
(5'-end capped and 3'-end poly[A] tailed) through the use of
CELLSCRIPT™'s ScriptCap™ Cap 1 Capping System (contains both ScriptCap™ Capping Enzyme and 2'-O-Methyltransferase) and A-Plus™ Poly(A) Polymerase Tailing Kit (available separately).
For reduction of immunogenicity, combine U-mRNA with the Min-Immune™ Gold dsRNA Removal Kit
to produce virtually dsRNA-free (to <0.005% of sample [LLOQ]),
ultra-low immunogenicity mRNA that is suitable for downstream
applications such as cell and gene therapy research and mRNA vaccine
development. CELLSCRIPT™ also offers the INCOGNITO™ line of products
to produce low immunogenicity mRNA containing the modified nucleotides
N1-methyl-pseudouridine, pseudouridine and/or 5-methyl-cytidine.
Product Performance:
The standard 30 minute, 20 μl T7-FlashScribe™ reaction was optimized
for transcription using 1 μg of linearized DNA template. However, higher
or lower amounts of DNA template can be used successfully with protocol
modifications. Table 1 summarizes IVT RNA yields from varying the input
amount of control template DNA and incubation time in T7-FlashScribe™
reactions. Results may vary depending on the template used.
Table 1. RNA yields from
T7-FlashScribe™ transcription reactions with varying DNA inputs and
incubation times. The standard protocol uses 1 µg of DNA input and 30
minute incubation time.
Materials Supplied:
Important Store at -20°C in a freezer without a defrost cycle. Do not store at -70°C.
T7 Control Template DNA: Is a linearized 4.1 kb
plasmid that contains a T7 promoter followed by a phage lambda dsDNA
insert that encodes a 1,375 base runoff transcript. The Control Template
DNA is provided at a concentration of 0.5 µg/µl in T10E1 Buffer (10 mM Tris-HCl, pH 7.5, 1 mM EDTA).
Materials Required, but not Supplied
- A DNA template for transcription of your RNA of interest
- Materials or kits for purification of the RNA product
- RNase-free TE Buffer (10 mM Tris-HCl, pH 7.5, 1 mM EDTA)
Downloads
Manual
SDS
Product Flyer
CELLSCRIPT™ RNA In vitro Transcription Kits Comparison Table
Terms and Conditions: The Product listed is currently offered by CELLSCRIPT™ for research use only under the defined Terms and Conditions.
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200