The PCR Decontamination Kit removes contaminating DNA in PCR master mixes, without reduction of PCR sensitivity.
Note: due to the wide variety of master mixes available, we recommend testing your master mixes for compatibility.
• The PCR Decontamination Kit removes contaminating DNA in PCR master mixes, without reduction of PCR sensitivity.*
• The double-strand specific property of the dsDNase allows decontamination with primers and probe present.**
• Efficient for end-point PCRs and probe based qPCR mixes, and for some SYBR based qPCR mixes.
• Contaminating bacterial DNA is reduced to levels below detection limit.
• Flat NTCs!
• The sensitivity of the PCR is not affected.
• Fast and easy protocol.
Kit Contents:
DTT (Inactivation Aid)
Cryophile dsDNase HL65
* Reduced PCR sensitivity can be observed in some kits, especially where hot-start is damaged upon inactivation of the dsDNase
*In some cases, primers and probes might form hairpin structures (not necessarily compatible) that might act as a target for the dsDNase. Hence, this must be tested from case to case. If this occurs, primers must be added after the decontamination.
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