Lab Reagents: Smart Buffers and Reagents
Biological buffers for the life science laboratory need to meet a wide array of criteria. These include good stability, lack of toxicity, precision in pH, pKa between 6.0 and 8.0 (the region in which most biological reactions occur), minimal salt effects due to the ionic composition of the solution, free of enzymatic and hydrolytic activity and minimal participation in biological reactions.
Buffers for molecular biology applications, e.g. Tris-Borate-EDTA and Tris-Acetate-EDTA, need to be free from DNAse and RNAse activity. Medicago's SmartBuffers meet all these criteria. They are manufactured using highly purified, analytical grade chemicals and are extensively quality tested. Manufacturing is done in a controlled clean environment according to GMP procedures.
A high level of automation essentially avoids operator intervention and contact with the products.
SmartBuffers are supplied as exactly pre-weighed tablets packed in containers or blister packs or as exactly pre-weighed powder mixes packed in sealed aluminium foil pouches.
•Pre-mixed powder buffers or tablets with pre-set pH
•Analytical grade reagents
•Eliminates calculation, formulation and weighing errors
•Dissolve-and-go for greater convenience
•Stable at room temperature for at least 3 years
•Guaranteed reproducibility
HEPES Buffered Saline (HBS) pH 7,4
HEPES is a routinely used buffer in cell culture, enzymology, chromatography and in general biochemistry.
Medicago AB
Description
Features
• Dissolve-and-go for greater convenience
• Exactly pre-weighed tablets in bottles or blister packs
• Guaranteed reproducibility
• Formulated from analytical grade chemicals
Applications
• Cell culture
• Enzymology
• Chromatography
• General Biochemistry
Description
HEPES is a routinely used buffer in cell culture, enzymology, chromatography and in general biochemistry.
Preparation
Dissolve one tablet in the specified
quantity of deionized water. This yields a solution containing 0.01 M
HEPES pH 7.4 and 0.15 M Sodium chloride.
Storage and stability
Tablets stable for 3 years at room temperature.
Associated products
PBS
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