mineralized tissue lining the surface of the tooth root, is required for
formation of a functional periodontal ligament attachment during
development. Additionally, during regeneration of tissues after disease,
cementum is thought to play a critical role in the reparative process.
Cementoblasts play a
central role in periodontal development and regeneration, and in
particular the formation of root cementum. Cementoblasts were first
isolated from OC-TAG transfenic mice in Martha Somerman lab in
University of Michigan, Ann Arbor. These mice contain a construct that
employed the SV-40 T antigen under the direction of the osteocalcin
(OCN) promoter. In this situation, when cells are isolated from the
tooth root surface of OC-TAG mice, only those cells that express
osteocalcin (i.e., cementoblasts) are capable of surviving serial
passages in vitro. The immortalized cementoblasts obtained in this
manner were subcloned, and one clone was designated as OCCM-30. These
cells were shown to exhibit the same properties as comparable cells
found in vivo, i.e., they expressed high levels of cementum-associated
markers, such as BSP and OCN, and promoted biomineralization in vitro
and in vivo.
The Transfection Reagent:
Cementoblast (OCCM-30 cell) Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on Cementoblasts (OCCM-30 cells). The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Cementoblasts (OCCM-30 cells). No other transfection reagents can match the efficiency, convenience, and gentleness of Cementoblast (OCCM-30 cell) Avalanche™ Transfection Reagent for transfection on Cementoblasts (OCCM-30 cells). For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
formulation-maximize transfection performance in Cementoblasts (OCCM-30
cells). More than 90% transfection efficiency as indicated by GFP
positive cells in flowcytometry analysis.
Extremely gentle to cells
Much less reagent needed for each transfection: 0.5 ml is able to transfect about 1000 wells of 24-well plate
Deliver single or multiple plasmids
Simplest and straightforward protocols
Compatible with serum
Compatible with transfection in any plate formats
Economical: High efficiency means less amount of nucleic acid & reagent is needed
|FIG. 1. High
throughput test of transfection efficiency (determined as RLU/mg) on
Cementoblasts (OCCM-30 cells) after transfection of luciferase reporter
gene by using our 172 proprietary transfection formulas and several most
popular commercial transfection reagents. The yellow box showed the
results of 4 commercial transfection reagents. The red lines marked our
candidate formulas with the highest transfection efficiency for
Cementoblasts (OCCM-30 cells). This test result was confirmed with
repeat experiments. The one that showed the optimal balance of potency
& low cytotoxicity among those candidate formulas after flow
cytometry analysis on the percentage of 7AAD positive cells was later
named as this A-375 Cell Avalanche Transfection Reagent.
|FIG. 2. OCCM-30 cells
were transfected with GFP vector (pEGFP-N3) by using OCCM-30 Cell
Avalanche® Transfection Reagent. The cells were visualized by Nikon
Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells:
(OCCM-30 cell) Avalanche™ Transfection Reagent can also be used on the
following cells with high transfection efficiencies.
Recommended protocols for these cells will be provided with the
reagent. The protocols usually provide satisfactory transfection
efficiency with invisible cytotoxicity. However, optimization may be
needed for certain type of cells. Optimizations may include: the amount
of DNA and this transfection reagent; cell density; transfection
reagent/DNA ratio, or incubation time for the mixture of transfection
reagent/DNA etc. For best transfection result, we recommend using the
respective cell type/cell line specific Avalanche transfection reagents.
Those reagents have been optimized on both recipes and protocols, and
have been proved to have the best transfection results for the
respective cell lines or primary cells. You can easily find the
respective Avalanche transfection reagents specific for your cells by
using the filters of our product list.
Zhao H, Qin X, Zhang Q, Zhang X, Lin J, Ting K, Chen F. Nell-1-ΔE, a novel transcript of Nell-1, inhibits cell migration by interacting with enolase-1. J Cell Biochem. 2018 Jul;119(7):5725-5733. doi: 10.1002/jcb.26756. Epub 2018 Apr 16. PubMed PMID: 29388706.
Zhang YY, Huang YP, Zhao HX, Zhang T, Chen F, et al. Cementogenesis is inhibited under a mechanical static compressive force via Piezo1. Angle Orthod. 2017 Jul;87(4):618-624. PubMed PMID: 28418701.
Zhang YY, Zhao HX, Chen ZB, Lin JX, Liu Y. Osteoprotegerin Promotes Cementoblastic Activity of Murine Cementoblast Cell Line in vitro. Chin J Dent Res. 2016 Jun;19(2):103-8. PubMed PMID: 27379348.