- Selectively react with primary aliphatic amine groups
- Preparation of oligos with fluorophores sensitive to basic deprotection conditions
ROX is a rhodamine dye that has played a significant role in dideoxy
Sanger sequencing alongside FAM, JOE and TAMRA. The dye itself is also
commonly used for qPCR applications as a passive reference dye. ROX is
not available as a phosphoramidite, and as such, the use of this NHS
ester is the standard method of incorporating ROX into oligonucleotides.
This NHS ester is offered as the 6-isomer.
F.W.:516.60
Usage
- Coupling:
For labeling 0.2 µmoles of an amino-modified oligonucleotide, dissolve
3.2 mg of the ROX NHS Ester in 100 µL DMSO (or DMF). Dissolve the
amino-modified oligonucleotide in 500 µL 0.1 M carbonate/bicarbonate
buffer (pH 9). Add 20 µL of the NHS Ester solution and vortex. Let react
for 60 min at room temperature and desalt.
| Specifications |
| Diluent |
DMF or DMSO |
| Storage | Freezer storage, -10 to -30°C, dry |
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