ROX NHS Ester -
6-Carboxy-X-rhodamine, N-hydroxysuccinimide ester
F.W.:516.60
Usage
- Coupling:
For labeling 0.2 µmoles of an amino-modified oligonucleotide, dissolve
3.2 mg of the ROX NHS Ester in 100 µL DMSO (or DMF). Dissolve the
amino-modified oligonucleotide in 500 µL 0.1 M carbonate/bicarbonate
buffer (pH 9). Add 20 µL of the NHS Ester solution and vortex. Let react
for 60 min at room temperature and desalt.
| Specifications |
| Diluent |
DMF or DMSO |
| Storage | Freezer storage, -10 to -30°C, dry |
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