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PCR

PCR : Reagents

Accessories for your PCR experiment

TATAA GrandScript cDNA FreePrime Kit

Robust and sensitive cDNA synthesis with user-defined RT priming

Tataa Biocenter

Catalogue No.DescriptionPack SizePriceQty
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  • £
AF103aTATAA GrandScript cDNA FreePrime Kit25 rxns POA Quantity Add to Order
AF103bTATAA GrandScript cDNA FreePrime Kit100 rxns POA Quantity Add to Order

Description

TATAA GrandScript cDNA FreePrime Kit

 

 

The TATAA GrandScript cDNA Freeprime Kit provides all the necessary components for cDNA synthesis conveniently separated into different tubes, giving the user freedom to setup the RT priming strategy suitable for your application.

The TATAA GrandScript cDNA Freeprime Kit provides a robust, consistent and unbiased first strand synthesis over a broad range of RNA template concentrations. The cDNA product is directly compatible with quantitative real-time PCR methods.

Random sequence and oligo(dT) primers are provided in separate tubes. User can also choose to use gene specific primers or Two-tailed primers, or a combination of the different priming strategies.

The TATAA GrandScript RT enzyme is a mixture of engineered M-MLV Reverse transcriptase and ribonuclease inhibitor.

Kit Contents

  • TATAA GrandScript FreePrime reaction mix (5x concentrated solution of optimised buffer, magnesium, dNTPs and stabilizers)
  • TATAA GrandScript RT enzyme
  • Oligo dT (10x conc. Solution of oligo(dT)20 with enhancer
  • Random primer (10x conc. Solution of random primer with enhancer)
  • GSP Enhancer
  • Nuclease-free water

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Protocols

Manual_TATAA GrandScript FreePrime_v1.0

 

Two-tailed RT-qPCR_a novel method for highly accurate miRNA quantification NAR 2017

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200