sbeadex™ blood kit
Next generation beads for DNA purification from blood
Blood samples can often vary considerably in quality, and this can
lead to wide variations in the consistency and yield of extracted DNA.
Molecular testing protocols, including genotyping and next-generation
sequencing applications require reliable and high quality sample
preparations.
DNA purification from blood protocol
sbeadex™ blood is a magnetic bead based DNA purification technology
for blood samples of up to 200 µL volume. These proprietary
superparamagnetic beads are double coated and include 2-binding steps
and follow the standard workflow.
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sbeadex™ blood kits have been developed as a high-throughput and
cost-effective DNA isolation method. It supports automated DNA
extractions using magnetic particle handlers (e.g. KingFisher™) as well as
liquid handlers.
Standard blood sample (anticoagulants) and preparations are:
- EDTA blood
- Heparin blood
- Citrate blood
- Buffy coats
|
Applications for sbeadex™ blood kits
- NGS: enzymatic fragmentation
- Library/Cloning: suitable for digestion and ligation
- qPCR/PCR: compatible with fluorescent BHQ probes and KASP chemistry
- Sanger Sequencing
|
Advantages of a double-coated sbeadex blood purification kit
- Efficient - purification of high molecular weight DNA from whole blood and buffy coat preparations
- High quality – consistent OD260/280 ≥ 1.8 ratio and an average OD260/230 1.9 ratio
- Easy to automate – magnetic microparticle handling offers flexibility
- Absence of PCR inhibitors – no organic solvents or chaotropic salts in final wash buffers.
- Convenient – all buffers supplied are ready to use
- Optimised protocols - Less than 1 hour to DNA
Blood DNA purification kit specifications
| Colour |
Dark brown |
| Magnetic bead structure |
Proprietary double-coated technology (competitor: single-coated) |
| Size and shape |
Irregular
Diameter: 80% 5 – 10 µm; all < 53 µm |
| Maximum capacity |
2 µg DNA / mg sbeadex particle |
| Binding material and technology |
2-step binding mechanism: first
silica and second DNA adaptor |
| DNA Quality |
Consistently high dsDNA content - Average 260/280: 1.8 and
260/230: 1.9 |
| Purification format |
Prepare in 1.5 mL tubes/96-well blocks |
| Automation |
KingFisher (lab validated); applicable with alternative open liquid handlers |
| Sample Prep Time |
Less than 1 hour from sample to purified DNA |
| Sample material |
Whole blood; buffy coat; livestock; forensics; plasmids; other human samples |
| Compatibility |
Anticoagulants: EDTA, Citrate, Heparin |
| Yield |
From 4 – 12 µg
Average yield 6.7 µg |
| Scale |
100 µL to 200 µL tested with no effect on yield with an elution range from 50 – 200 µL |
| Purify from a range of blood storage conditions |
Within 8 hours of blood collection and up to 3 years (-20 ° C) in storage |
| Applications |
Suitable for
- digestion, ligation, cloning, PCR, qPCR
- NGS: Enzymatic fragmentation library pre-prep and size selection beads
Compatible with KASP®/qPCR/BHQ® Probe MMx and BHQ Probe |
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200