The sbeadex™ plasmid kit
The sbeadex™ plasmid kit has been developed for high throughput extraction of plasmid DNA from bacterial cultures. The magnetic particle based DNA extraction protocol can be easily automated using a KingFisher 96 (Thermo Fisher Scientific) magnetic particle manipulator. Using magnetic rods, which are protected against contamination by a tip comb. The magnetic beads are transferred from one buffer plate to the next during the extraction process. This instrument can process up to 96 samples per run. In addition, the KingFisher 96 instrument is compatible with liquid handling systems and sample handling devices, thus making a hands-free medium to high throughput system a reality.
sbeadex™ coated magnetic particles bind DNA using a novel two-step binding mechanism in the presence of detergents and salts. After binding and washing steps, the purified DNA is released in the elution buffer. The sbeadex® plasmid kit is supplied with ready-to-use buffers. The processing time with KingFisher 96 is approximately 20 minutes. Typically, plasmid DNA extraction from a 1 mL bacterial culture with KingFisher 96 and sbeadex® plasmid kit results in about 5 - 10 μg of high quality plasmid DNA.
For Protocol, kit contents, storage temperatures and additional items required please click here
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