P51™ Introduction to Fluorescence Lab: Glow Big or Glow Home
This lab serves as an introduction to the phenomenon of fluorescence
and its applications in biotechnology. It also provides an engaging
framework to develop foundational lab skills such as micropipetting and
making serial dilutions, through which students create a standard curve
to estimate unknown solute concentrations. Students will explore the
properties of fluorescein, a commonly used indicator in biotechnology
applications. By taking an inquiry-based approach, students will grow
comfortable using indicator molecules to answer experimental questions.
Go fluorescein glow!
- Techniques: Micropipetting, serial dilutions, fluorescence detection
- Topics: Fluorescence, biotechnology, concentrations, scientific notation
- Time required: Can be completed in one 45-minute period
- Level: General high school through advanced high school
The P51™ Introduction to Fluorescence Lab kit contains reagents for 8 lab groups:
- Concentrated Fluorescein
- Dilution Buffer
- Unknown Sample
- 200 μl strip-tubes
- Individual 200 μl tubes
- Microcentrifuge tubes
*Note: Reagents are compatible with P51™ Molecular Fluorescence Viewer or other blue light transilluminator (460-480 nm, e.g. blueBox™ or blueGel™).
Required equipment (one per lab group):
Storage notes:
- Reagents can be stored at room temperature, protected from light
- Reagents are stable for 24 months under recommended conditions
Downloads
SDS – Concentrated Fluorescein
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