This kit provides a specific primer/probe mix and offers an
open-platform solution for use with various dPCR devices (QIAcuity®,
ddPCR™, Quantstudio™ Absolute Q™). MiQuant® CAR-T Cell Lenti – dPCR
works for all HIV-1 based lentivirus transduction systems.
Type of PCR
Digital PCR (dPCR)
Kit Components
- MiQuant® Lenti Mix (lyophilized)
- Positive Control DNA (lyophilized)
- dPCR grade Water
Package Size
Cat. No. 58-0701: 35 µl primer/probe mix
Cat. No. 58-0702: 140 µl primer/probe mix
Since every dPCR device requires different PCR reaction volumes, the number of PCR reactions are shown in the following table.
Result Evaluation
Absolute Quantification
Required Lab Device and Consumables
- Digital PCR device with corresponding consumables (plates or stripes)
- QIAcuity®
- Quantstudio™ Absolute Q™
- QX600™ or QX200™ ddPCR™
- Corresponding Mastermix (QIAcuity® Probe PCR Kit (4x), Absolute Q™
DNA Digital PCR Master Mix (5x), ddPCR™ Multiplex Supermix (4x) or
ddPCR™ Supermix for Probes (2x))
- MiQuant® SP Beads (sample preparation kit, Cat. No. 56-4010/-4100)
- 1.5 ml reaction tubes (low-bind, DNA- and RNA-free)
- Microcentrifuge for 1.5 ml PCR tubes
- Vortex mixer
- Pipettes with corresponding filter tips
Optional:
- Restriction enzymes (EcoRI-HF and NruI-HF; 20 U/μl)
- PCR Clean™ (Cat. No. 15-2025 and 15-2001)
Shelf Life and Storage
Shipment at ambient temperature. The expiry date of the unopened
package is marked on the package label. The kit components must be
stored at 2 to 8°C until use. The rehydrated components must be stored
at ≤ -18°C for a maximum of one month.
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200