NxGrade Custom Oligonucleotides
Custom NGS-grade oligos designed to improve library-prep
success and minimize index crosstalk in multiplexed sequencing
workflows.
Key benefits- High quality at great value: Oligos with high purity and reproducibility without the added cost of HPLC purification.
- Low cross-contamination: Consistently low oligo
cross-contamination to reduce barcode crosstalk and improve data
integrity in multiplexed NGS experiments.
- Flexible formulation options: Choose from single, paired, or annealed oligos to fit seamlessly into your workflow.
- Dedicated support for your larger projects: Work
with our collaborative team to develop and deliver high-volume or
commercial products tailored to your exact specifications.
Our NxGrade™ custom oligonucleotides production line is purpose-built for NGS indexing primers and adapters.
Our supplier, LGC Biosearch Technologies, operates a dedicated production line
with an optimized workflow specifically engineered to deliver oligos
with high purity, excellent reproducibility, and maximal
cross-contamination mitigation. Their NxGrade oligo production line is
routinely monitored using NGS-based testing to help ensure effective
cross-contamination control.
| Cross-contamination levels* |
| <0.05% |
*Typical levels determined by routine in-house NGS monitoring.
Low oligo cross-contamination in NGS indexing primers and adapters is
essential for reliable sequencing results because even trace amounts of
the wrong index can cause barcode misassignment, where reads are
assigned to the wrong sample. Cross-contaminated or low-purity oligos,
containing truncated sequences, can contribute to adapter crosstalk or
index hopping. These issues can degrade data integrity, compromise
downstream analysis, cause sample misidentification, waste sequencing
capacity, delay projects, and increase overall costs.
Traditional HPLC purification methods reliably generate high-purity,
full-length oligos, but they can also introduce cross-contamination from
previously purified sequences.
LGC's optimized reverse-phase cartridge (RPC) purification method
offers a high-value alternative, producing oligos with high purity while
minimizing cross-contamination. RPC purification effectively removes
truncated species and other impurities, without the contamination risks
commonly associated with HPLC.
Specifications
| Sequence length range |
10–80 bases |
| Purification type |
Optimized reverse-phase cartridge (RPC) |
| Standard delivered amounts |
Small: 5 nmol yield per oligo
Medium: 20 nmol yield per oligo
Large: 50 nmol yield per oligo |
| Modifications |
| 5’ modifications |
5’ phosphate |
| Internal modifications and bases |
Phosphorothioate bond
Degenerate bases |
|
| Delivery format |
1 or 2 oligos per well, annealing optional
Dry or in solution |
| Vessels |
Deep well 96-well plate
Racked Matrix™ tubes |
| Cross-contamination level |
<0.05%* |
| Quality control |
100% mass spectrometry
Analytical UPLC |
| Quality management system |
ISO 9001 |
*Typical cross-contamination levels are determined by routine NGS monitoring.
Flexible Formulation Options
Flexible custom formulation options for high-volume projects
Our dedicated, collaborative team works closely with you to customize
formulation, delivery format, and labeling to meet the exact needs of
your project.
Our capabilities include:
- Bulk oligo production in plate format: quantities up to 100s of nmol per sequence, with a wide range of available modifications.
- Versatile delivery formats: 96-well or 384-well PCR plates, deep-well plates, or Matrix tubes.
- Ready-to-use plate options: stamped, single-use plates with heat seals for streamlined workflows.
- Custom labeling: tailored labeling to support seamless integration into your tracking and LIMS systems.
- Controlled manufacturing environment: formulations produced in an ISO 13485-certified, non-oligo synthesis facility.
- Collaborative QC development: partner with us to design and validate in-process and final QC methods, including QC plates and outgoing NGS QC.
Seeking a partner for your next project?
We are ready to assist:
Let us know what you are trying to achieve and our
expert team will be in contact within a business day to talk through
your needs. contact us on support@cambio.co.uk or use the contact us page
Resources
Storage and Handling of Primers,
Custom Oligos, & BHQ Probes
SDS for unlabeled oligos
Product flyer
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200