RNase inhibitors are commonly used to inhibit and control RNase contaminants in enzymatic manipulations of RNA. Intact Genomics (ig®) Glycerol-free RNase Inhibitor is a 49.8 KDa recombinant protein of murine origin which specifically inhibits RNases A, B and C by binding noncovalently in a 1:1 ratio with high affinity. Moreover, it does not inhibit polymerase activity when used with Taq DNA Polymerase, Reverse Transcriptase. Glycerol-free RNase Inhibitor from Murine is resistant to oxidation compared to the human/porcine RNase inhibitors as it lacks the pair of cysteine residues found in the human/porcine versions. In addition, it is stable at less than 1 mM DTT concentration.
Benefits of Glycerol-Free Enzyme:
- Optimal for Lyophilization: Perfect for freeze-drying into diagnostic kits, ensuring stability and performance for point-of-care tests like molecular diagnostics.
- Better Compatibility: Avoid interference in sensitive applications where glycerol limits solubility in certain organic solvents.
- Improved Performance: Enzyme formulations are easier to handle in high-throughput assays, as there’s no added viscosity affecting pipetting.
- Simplified Integration: Allow for more flexible formulation, making it easier to add specific stabilizers or buffers for optimized performance in various systems.
Protein Source:
1. coli strain expressing recombinant RNase Inhibitor gene from mouse.
Applications:
- cDNA synthesis
- RT-PCR/RT-qPCR
- In vitro transcription/translation
- RNA labeling
- Microfluidics assays
Protein Purity:
The physical purity of this enzyme is ≥98% as assessed by SDS-PAGE with Coomassie® blue staining.
Product Components & Storage:
- RNase Inhibitor, Murine, Glycerol-free : -80°C
Enzyme Storage Buffer
- 50 mM HEPES, 750 mM KCl, 1 mM DTT, and 0.1 mM EDTA, pH 7.5
Heat Inactivation:
70ºC for 20 minutes
Unit Definition:
One unit is defined as the amount of RNase Inhibitor is required to inhibit the activity of 5 ng of RNase A by 50%.
Quality Control:
SDS-PAGE purity, protein concentration, endodeoxyribonuclease assay, performance evaluation with RT-qPCR with activity meeting or exceeding reference enzyme.
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200