RNase inhibitors are commonly used to inhibit and control RNase contaminants in enzymatic manipulations of RNA. Intact Genomics RNase Inhibitor is a 49.8 KDa recombinant protein of murine origin which specifically inhibits RNases A, B and C by binding noncovalently in a 1:1 ratio with high affinity. Moreover, it does not inhibit polymerase activity when used with Taq DNA Polymerase, Reverse Transcriptase. This Inhibitor from Murine is resistance to oxidation compared to the human/porcine inhibitors as it lacks the pair of cysteines found in the human/porcine versions. In addition, it is stable at less than 1 mM DTT concentration.
Protein Purity
The physical purity of this enzyme is ≥98% as assessed by SDS-PAGE with Coomassie® blue staining (Fig. 1).
Product Source
E. coli strain expressing recombinant RNase Inhibitor gene from mouse.
Product Includes
Applications
- cDNA synthesis
- RT-PCR/RT-qPCR
- In vitro transcription/translation
- RNA labeling
Unit Definition
One unit is defined as the amount of the Murine Inhibitor is required to inhibit the activity of 5 ng of RNase A by 50%.
Storage Temperature
–20ºC
Heat Inactivation
70ºC for 20 minutes
Storage Buffer
50 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.5 @ 25ºC
Storage Temperature
–20ºC
Quality Control assays
SDS-PAGE purity, protein concentration, endo-deoxyribonuclease assay, performance evaluation with RT-qPCR
RNase Inhibitor (RI) Data Comparison
RT-qPCR was run using Intact Genomics (IG) 1-step 2x master mix (MM) and compared IG RI with a leading brand’s “N” RI.
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