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Isothermal Nucleic Acid Amplification

Isothermal Nucleic Acid Amplification: RPA Optimization Enzymes & Kits

Isothermal Nucleic Acid Amplification Kits, Enzymes, and Lysis Solutions

 

Since 2016, Intact Genomics has pioneered the development and manufacture of products for isothermal nucleic acid amplification. Our comprehensive portfolio includes RPA master mixes and kits, individual RPA enzymes, glycerol-free and lyophilized formats, FastAmp® Viral and Cell Lysis Solutions, and helicase-based amplification enzymes. Learn more by downloading our white paper on RPA technology and Intact Genomics RPA products.

Designed for rapid, sensitive nucleic acid amplification without conventional thermal cycling, our products support molecular diagnostic development, point-of-care and agricultural testing, and life science research. Through continued innovation, consistent quality, and reliable performance, we help researchers and industry partners accelerate discovery and develop next-generation testing solutions. Bulk quantities, custom formulations, CDMO and OEM services are also available.

Product Links

Package Size

Features

IG® RPA Master Mix

25 rxns, 100 rxns, 500 rxns

Delivers powerful isothermal amplification for molecular diagnostics and research. Easy-to-use, fast, reliable, and cost-effective.

IG® RPA Master Mix - Lyophilized(opens in new tab)

24 rxns, 96 rxns, 480 rxns

A ready-to-use, freeze-dried premix for rapid and sensitive isothermal DNA amplification.

IG® Recombinase Polymerase Amplification (RPA) Kit v2

25 rxns, 100 rxns, 500 rxns

Provides faster and more discernible results for isothermal DNA amplification.

FastAmp® qRPA SYBR Kit

25 rxns, 100 rxns, 500 rxns

Screens primer conditions to minimize non-template control (NTC) background.

FastAmp® Plant Direct RPA Kit

25 rxns, 100 rxns, 500 rxns

Rapid DNA amplification from plant tissues at a constant temperature of 37°C.

IG® Recombinase Polymerase Amplification (RPA) Kit

25 rxns, 100 rxns, 500 rxns

Contains the reagents and enzymes needed for DNA amplification.

T4 UvsX DNA Recombinase(opens in new tab)

100 μg, 500 μg, 1000 μg

Important for error-free repair of DNA double-strand breaks and replication fork restart.

T4 UvsY Protein

100 μg, 500 μg, 1000 μg

Phage T4 recombination mediator protein that stimulates filament nucleation.

T4 gp32 Protein

100 μg, 200 μg, 500 μg

Single-stranded DNA-binding protein involved in DNA replication, recombination, and repair.

Bsu DNA Polymerase Large Fragment

1,000 units

Polymerase useful for extending primers in RPA.

Sau DNA Polymerase Large Fragment

1,000 units

Polymerase useful for extending primers in RPA.

Glycerol Free or Lyophilized T4 UvsX DNA Recombinase

100 μg, 500 μg, 1000 μg

Supports error-free DNA double-strand break repair and replication fork restart.

Glycerol Free or Lyophilized T4 UvsY Protein

100 μg, 500 μg, 1000 μg

Recombination mediator protein that stimulates filament nucleation.

Glycerol Free or Lyophilized T4 gp32 Protein

500 μg

Single-stranded DNA-binding protein for DNA replication, recombination, and repair.

Glycerol Free or Lyophilized Bsu DNA Polymerase Large Fragment

1,000 units

Polymerase useful for extending primers in RPA.

Exonuclease III

10,000 units, 25,000 units

Digests one strand of duplex DNA from blunt ends, 5′ overhangs, or nicks.

Endonuclease IV (Nfo)

2,000 units, 5,000 units

Functions as an apurinic/apyrimidinic nuclease and 3′-terminal di-esterase for DNA repair.

FastAmp® Viral and Cell Lysis Solution (1×, 5×)

25 reactions, 100 reactions

Enables direct use of lysed DNA or RNA in RPA, RT-isothermal amplification, PCR, and RT-qPCR without extraction.

Tte MutL Protein(opens in new tab)

(opens in new tab)20 µg, 400 µg

A thermostable ATPase accessory protein that interacts with Tte UvrD helicase, suitable for mismatch repair studies, helicase regulation, and isothermal DNA amplification development.

Tte UvrD Helicase(opens in new tab)

2 µg, 40 µg

A thermostable, ATP-dependent DNA helicase and key enzyme for HDA, suitable for isothermal nucleic acid amplification, DNA-unwinding studies, helicase activity assays, and primer- or probe-based detection workflows.

igSuper™ Tte UvrD Helicase(opens in new tab)

(opens in new tab)2 µg, 40 µg

An engineered, thermostable ATP-dependent DNA helicase for HDA and other isothermal nucleic acid amplification, DNA-unwinding studies, and DNA detection workflows.

 

IG® RPA Master Mix

Fast, reliable, and easy-to-use — the IG® RPA Master Mix (patent pending) delivers powerful isothermal amplification for your molecular diagnostics and research.

Intact Genomics

Catalogue No.DescriptionPack SizePriceQty
  • Change to:
IG-3545IG® RPA Master Mix25 Reactions �217.77 Quantity
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IG-3547IG® RPA Master Mix 100 Reactions  �479.57 Quantity
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IG-3549IG® RPA Master Mix500 Reactions  �1,600.55 Quantity
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IG® RPA Master Mix

 

 

Fast, reliable, and easy-to-use — the IG® RPA Master Mix (patent pending) delivers powerful isothermal amplification for your molecular diagnostics and research.

  • Works at 25–37°C — no thermal cycler needed
  • More affordable and accessible
  • Optimized for high sensitivity and consistent results
  • Simplify your workflow. Amplify your results

Custom formats, package sizes and bulk pricing are available upon request.

 

IG® RPA Master Mix is a ready to use premix which contains T4 UvsX, UvxY, gp32, Bsu DNA polymerase, and stabilizers with an optimized reaction solution. It simplifies and quickens the setup for your RPA reaction. This patent pending, proprietary IG® RPA Master Mix offers two key advantages over other RPA products: 1) More robust amplification at shorter times and 2) Lower background amplification of off-target products meaning IG® RPA Master Mix has more specific amplification of the primary target.

Figure 1: IG RPA Master Mix amplified DNA in 10 minutes at 25°C, in 5 minutes at 37°C.

Key Features:
•  Rapid isothermal DNA amplification at 25 °C
•  Results in as little as 5–10 minutes
•  Low background and reduced nonspecific amplification
•  Detection sensitivity below 1,000 copies per reaction
•  No thermal cycler required
•  Suitable for laboratory and field-deployable assays
•  Convenient lyophilized format available

Comparison of Intact Genomics (IG) Lyophilized RPA Master Mix (MM) vs ThermoFisher (TF) Lyo-ready RPA kit:
In recent years, Thermo Fisher Scientific (TF) launched its Lyo-ready RPA kit and published misleading data on its website comparing an Intact Genomics (IG) RPA kit with TF’s Lyo-ready RPA kit. We have no record of a TF scientist purchasing our RPA kit, and TF did not specify which IG RPA product was used in its comparison. In response, we purchased TF’s Lyo-ready RPA kit (Cat. #A72127) and conducted a side-by-side comparison with the IG Lyophilized RPA Master Mix (Cat. #3545Lyo).
RPA Activity Results:

Lane 1. IG RPA MM: 88 pg pUC19 DNA, 214 bp target.
Lane 2. TF RPA Kit: 88 pg pUC19 DNA, 214 bp target.
Lane 3. IG RPA MM: 1 ng soybean gDNA, 321 bp universal target.
Lane 4. TF RPA Kit: 1 ng soybean gDNA, 321 bp universal target.
Lane 5. IG RPA MM: 1 ng soybean gDNA, 242 bp low-copy SMC1 target.
Lane 6. TF RPA Kit: 1 ng soybean gDNA, 242 bp low-copy SMC1 target.

Findings Under the Tested Conditions:

  • The TF Lyo-ready RPA Kit showed no detectable amplification at 25 °C after 30 minutes, even with the pUC19 plasmid template.
  • At 37 °C after 20 minutes, the TF kit showed no detectable amplification of the soybean low-copy SMC1 target (lane 6) and only faint bands with other targets.
  • The IG Lyophilized RPA Master Mix amplified all targets at 37 °C within 20 minutes.
    At 25 °C after 30 minutes, the low-copy SMC1 target was not detected with 1 ng of soybean genomic DNA.
  • IG’s Lyophilized RPA Master Mix requires 3 pipetted additions plus 1 lyophilized vial, versus 10 liquid additions for TF. In a 20 µL reaction, the smallest transfer is 1 µL for IG versus 0.4 µL for TF.

RPA Reaction Set up:

Click here to download the comparison results as a PDF.

Applications:

  1. Healthcare and Infectious Disease Diagnostics – Rapid detection of pathogens such as SARS-CoV-2, HIV, influenza, and tuberculosis.Decentralized testing in clinics, emergency settings, and resource-limited areas.
  2. Food Safety – On-site detection of foodborne pathogens (e.g., Salmonella, E. coli).Verification of allergen contamination and quality control.
  3. Agriculture – Early detection of plant pathogens and crop diseases.Monitoring livestock infections.
  4. Environmental Monitoring – Detection of microbial contamination in water supplies.Biosurveillance of emerging pathogens in ecosystems.
  5. Biodefense and Security – Rapid field-deployable assays for detection of biothreat agents.

Specifications:

Enzymes: Nuclease free, >95% purity by Coomassie stained polyacrylamide gel analysis.
Format: Tubes
Shipping Condition: Dry ice & frozen gel packs

Product Components:

  • IG® RPA Master Mix
  • RPA Reaction Starter

Storage:

  • IG® RPA Master Mix: -20 °C
  • RPA Reaction Starter: 4 °C or -20 °C

Quality Control:

All enzymes thoroughly tested for activity and purity.
All component proteins of the Enzyme Mixture are free from detectable nuclease activities.
Product quality manage system is ISO 13485 certified.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200