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Isothermal Nucleic Acid Amplification

Isothermal Nucleic Acid Amplification: RPA Optimization Enzymes & Kits

Isothermal Nucleic Acid Amplification Kits, Enzymes, and Lysis Solutions

 

Since 2016, Intact Genomics has pioneered the development and manufacture of products for isothermal nucleic acid amplification. Our comprehensive portfolio includes RPA master mixes and kits, individual RPA enzymes, glycerol-free and lyophilized formats, FastAmp® Viral and Cell Lysis Solutions, and helicase-based amplification enzymes. Learn more by downloading our white paper on RPA technology and Intact Genomics RPA products.

Designed for rapid, sensitive nucleic acid amplification without conventional thermal cycling, our products support molecular diagnostic development, point-of-care and agricultural testing, and life science research. Through continued innovation, consistent quality, and reliable performance, we help researchers and industry partners accelerate discovery and develop next-generation testing solutions. Bulk quantities, custom formulations, CDMO and OEM services are also available.

Product Links

Package Size

Features

IG® RPA Master Mix

25 rxns, 100 rxns, 500 rxns

Delivers powerful isothermal amplification for molecular diagnostics and research. Easy-to-use, fast, reliable, and cost-effective.

IG® RPA Master Mix - Lyophilized(opens in new tab)

24 rxns, 96 rxns, 480 rxns

A ready-to-use, freeze-dried premix for rapid and sensitive isothermal DNA amplification.

IG® Recombinase Polymerase Amplification (RPA) Kit v2

25 rxns, 100 rxns, 500 rxns

Provides faster and more discernible results for isothermal DNA amplification.

FastAmp® qRPA SYBR Kit

25 rxns, 100 rxns, 500 rxns

Screens primer conditions to minimize non-template control (NTC) background.

FastAmp® Plant Direct RPA Kit

25 rxns, 100 rxns, 500 rxns

Rapid DNA amplification from plant tissues at a constant temperature of 37°C.

IG® Recombinase Polymerase Amplification (RPA) Kit

25 rxns, 100 rxns, 500 rxns

Contains the reagents and enzymes needed for DNA amplification.

T4 UvsX DNA Recombinase(opens in new tab)

100 μg, 500 μg, 1000 μg

Important for error-free repair of DNA double-strand breaks and replication fork restart.

T4 UvsY Protein

100 μg, 500 μg, 1000 μg

Phage T4 recombination mediator protein that stimulates filament nucleation.

T4 gp32 Protein

100 μg, 200 μg, 500 μg

Single-stranded DNA-binding protein involved in DNA replication, recombination, and repair.

Bsu DNA Polymerase Large Fragment

1,000 units

Polymerase useful for extending primers in RPA.

Sau DNA Polymerase Large Fragment

1,000 units

Polymerase useful for extending primers in RPA.

Glycerol Free or Lyophilized T4 UvsX DNA Recombinase

100 μg, 500 μg, 1000 μg

Supports error-free DNA double-strand break repair and replication fork restart.

Glycerol Free or Lyophilized T4 UvsY Protein

100 μg, 500 μg, 1000 μg

Recombination mediator protein that stimulates filament nucleation.

Glycerol Free or Lyophilized T4 gp32 Protein

500 μg

Single-stranded DNA-binding protein for DNA replication, recombination, and repair.

Glycerol Free or Lyophilized Bsu DNA Polymerase Large Fragment

1,000 units

Polymerase useful for extending primers in RPA.

Exonuclease III

10,000 units, 25,000 units

Digests one strand of duplex DNA from blunt ends, 5′ overhangs, or nicks.

Endonuclease IV (Nfo)

2,000 units, 5,000 units

Functions as an apurinic/apyrimidinic nuclease and 3′-terminal di-esterase for DNA repair.

FastAmp® Viral and Cell Lysis Solution (1×, 5×)

25 reactions, 100 reactions

Enables direct use of lysed DNA or RNA in RPA, RT-isothermal amplification, PCR, and RT-qPCR without extraction.

Tte MutL Protein(opens in new tab)

(opens in new tab)20 µg, 400 µg

A thermostable ATPase accessory protein that interacts with Tte UvrD helicase, suitable for mismatch repair studies, helicase regulation, and isothermal DNA amplification development.

Tte UvrD Helicase(opens in new tab)

2 µg, 40 µg

A thermostable, ATP-dependent DNA helicase and key enzyme for HDA, suitable for isothermal nucleic acid amplification, DNA-unwinding studies, helicase activity assays, and primer- or probe-based detection workflows.

igSuper™ Tte UvrD Helicase(opens in new tab)

(opens in new tab)2 µg, 40 µg

An engineered, thermostable ATP-dependent DNA helicase for HDA and other isothermal nucleic acid amplification, DNA-unwinding studies, and DNA detection workflows.

 

Sau DNA Polymerase Large Fragment

Sau DNA Polymerase is a robust strand-displacing enzyme designed for efficient DNA synthesis under isothermal conditions. Its strong polymerase and strand-displacement activities make it well suited for isothermal amplification, molecular diagnostics, and a broad range of nucleic acid research applications.

Aline Biosciences

Catalogue No.DescriptionPack SizePriceQty
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IG-3592Sau DNA Polymerase Large Fragment200 Units (5 units/µl) �182.07 Quantity
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IG-3595Sau DNA Polymerase Large Fragment1000 Units (5 units/µl) �479.57 Quantity
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Sau DNA Polymerase Large Fragment

 

 

Sau DNA Polymerase is a robust strand-displacing enzyme designed for efficient DNA synthesis under isothermal conditions. Its strong polymerase and strand-displacement activities make it well suited for isothermal amplification, molecular diagnostics, and a broad range of nucleic acid research applications.

 

Product Description

Sau DNA Polymerase I, Large Fragment is a product of the Staphylococcus aureus DNA polymerase I which lacks the N-terminal exonuclease domain (1-293 amino acids). It retains the 5´→ 3´ polymerase activity of DNA polymerase I but lacks the 5´→ 3´ exonuclease activity. This large fragment also lacks 3´→ 5´ exonuclease activity.

Applications

  • Strand displacement DNA synthesis (1)
  • Random primer labeling
  • Second strand cDNA synthesis
  • dA-tailing

Protein Purity

The physical purity of this enzyme is ≥99% as assessed by SDS-PAGE with Coomassie® blue staining (see figure below).

 

Product Source

E. coli strain expressing Sau Polymerase I gene lacking the N-terminal exonuclease domain.

Product Includes

• Sau DNA Polymerase I, Large fragment

• 10x Sau DNA Polymerase I reaction buffer

1x Sau DNA Polymerase I reaction buffer

10 mM Tris-HCl, 50 mM KCl, 10 mM MgCl2, 1 mM DTT, pH 7.9 @ 25°C

Storage Buffer

50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25ºC

Storage Temperature
–20ºC

Heat inactivation
70°C for 20 min

Unit Definition
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 37º C.

Quality Control Assays

Sau Polymerase I, Large fragment is free from detectable nuclease activities.  Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product.

References

1. Piepenburg, O. et al. (2006) PLOS Biology, 4, 1115–1121.

 

Manual – Sau DNA Polymerase Large Fragment

MSDS – Sau DNA Polymerase Large Fragment


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