The AquaScreen^® product line is an in vitro test system for quantitative detection of various water pathogens. The AquaScreen^® FastExtract kit is specifically designed for extracting microbial DNA from water samples. The kit complies with the design requirements of ISO/TS 12869:2012. The extracted DNA is used in conjunction with our AquaScreen^® qPCR kits for sensitive and reliable detection of legionella and other bacteria in water samples.

The AquaScreen^® FastExtract kit works robustly with various volumes of water samples by utilizing 0.4 µm filter membranes in order to capture microorganisms. Bacterial cells are subsequently lysed on the filter membrane, followed by spin column purification. The DNA is eluted in minimal volumes and ready-to-use for qPCR.

Features

Description Rapid DNA extraction from water samples

Scope of Application AquaScreen^® FastExtract can be used with your established suction device (47 mm frit) for the extraction of legionella and other microbial contaminations. AquaScreen^® FastExtract is optimized for high flow and throughput and provides high quality DNA for subsequent PCR analysis.

Kit Components Membrane filters Incubation dishes Incubation, collection and sample storage tubes Lysis, wash and elution buffers

Package Sizes Cat. No. 32-1010    10 extractions Cat. No. 32-1050    50 extractions

Required Lab Equipment & Consumables Vacuum pump Microcentrifuge Filtration system, 47 mm frit Pipets and filter tips Incubator (37 °C for petri dishes, 56 °C for reaction tubes) Ethanol (96-100 %)

Storage The kit must be stored at room temperature.

Compliance AFNOR XP T90-471 and ISO/TS 12869:2012 in combination with AquaScreen^® qPCR kits

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AquaScreen® Fast Extract Protocol

 Please note: all protocols off site are the responsibility of the products supplier

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Felföldi T. et al., (2010). Detection of potentially pathogenic bacteria in the drinking water distribution system of a hospital in Hungary. Clinical Microbiology and Infection, 16: 89–92 10.1111/j.1469-0691.2009.02795.x.

Kirschner A.K. (2016). Determination of viable legionellae in engineered water systems: Do we find what we are looking for?

Water Research, 93:276-88. doi: 10.1016/j.watres.2016.02.016.

Rozwadowska B. et al., (2013). Application of a real-time PCR method for Salmonella spp., Escherichia coli, Staphylococcus aureus and Clostridium perfringens detection in water samples. Polish Journal of Microbiology, 62(4):439-43.

Vogel J. et al., (2013). Impacts of Migratory Sandhill Cranes (Grus canadensis) on Microbial Water Quality in the Central Platte River, Nebraska, USA. Water, Air and Soil Pollution, 224:1576. doi: 10.1007/s11270-013-1576-3.

Yaradou D.F. et al., (2007). Integrated real-time PCR for detection and monitoring of Legionella pneumophila in water systems. Applied and Environmental Microbiology, 73(5):1452-6. doi: 10.1128/AEM.02399-06.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

• ViewAquascreen Frequently Asked Questions

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200