Applications

In vitro Transcription: In Vitro Transcription

ScriptCap™ m7G Capping System

For post-transcriptional capping of in vitro transcribed RNA with ScriptCap Capping Enzyme, GTP and SAM. Cap 0 (N7-methyl-G) caps can be built with efficiencies approaching 100%. This cannot be accomplished with co-transcriptional capping methods. Use in conjunction with ScriptCap 2'-O-Methyltransferase to produce Cap 1 caps, that which is found on most eukaryotic mRNAs.

CellScript

Catalogue No.	Description	Pack Size	Price	Qty	Change to: €
C-SCCE0625	ScriptCap™ m⁷G Capping System	25 Reactions	£350.00	Quantity	Add to Order

Description

ScriptCap™ m7G Capping System catalyzes in vitro addition of a cap nucleotide to the 5' terminus of primary RNA that has a 5'-triphosphate group, such as RNA obtained from an in vitro transcription reaction. A "cap nucleotide" or “cap” is a guanine nucleoside that is joined via its 5' carbon to a triphosphate group that is, in turn, joined to the 5' carbon of the most 5' nucleotide of the primary mRNA transcript, and in most eukaryotes, the nitrogen at the 7 position of guanine in the cap nucleotide is methylated. Such a capped transcript can be represented as:

m7G(5')ppp(5')N1(pN)x-OH(3')

where m7G represents the 7-methylguanosine cap nucleoside, ppp represents the triphosphate bridge between the 5' carbons of the cap nucleoside and the first nucleotide of the primary RNA transcript, and N1(pN)x-OH(3') represents the primary RNA transcript, of which N1 is the most 5' nucleotide.