• Achieve ~100% Cap 1 capping of Cap-0 RNA from any source, a level unattainable using cap analogs.
• Enhance mRNA stability and translation efficiency through post-transcriptional 5’ capping.
• Use simultaneously with ScriptCap™ m⁷G Capping System to produce Cap 1 caps in a single reaction.
• Simplified workflow with direct input of capped RNA into A-Plus™ Poly(A) Polymerase tailing reactions with no cleanup required.

Product is for research use only (RUO) 

Product Description:

The ScriptCap™ 2′-O-Methyltransferase Kit provides conversion of Cap 0-RNA from any source to Cap 1-RNA with ~100% efficiency, a level that cannot be obtained using co-transcriptional capping methods. 5’ Capping enhances mRNA stability and translation efficiency in cells compared to uncapped RNA, with Cap 1 methylation further boosting in vivo translation efficiency. A standard reaction methylates 60 μg of Cap 0-RNA and can be scaled up or down to accommodate user needs.

Cap 0-RNA can be produced by enzymatic capping using the ScriptCap™ m⁷G Capping System or by in vitro transcription of a DNA template in the presence of a dinucleotide cap analog (e.g., using MessageMAX™ T7 ARCA-Capped Message Transcription Kit V2). Uncapped RNA can be converted to Cap 1-RNA in a single reaction by combining the ScriptCap™ m⁷G Capping System and 2’-O-Methyltransferase.

ScriptCap™ capped RNA can be added directly to A-Plus™ Poly(A) Polymerase tailing reactions without cleanup for seamless synthesis of fully 5’ capped and 3’ tailed mRNA.

Materials Supplied:

Important Store at -20°C in a freezer without a defrost cycle. Do not store at -70°C.

Materials Required, but not Supplied

• Cap 0 RNA substrate
• Materials or kits for purification of the RNA product