Catalog Number: 20-2017-xx
(20-2117-xx)
Description: 2',3'-ddC-CPG
| 5'-Dimethoxytrityl-N-succinoyl-long chain alkylamino-CPG, 2',3'-deoxyCytosine |
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F.W.: 273.19 |
Diluent: Not Applicable |
Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.
Carry out the cleavage of the oligonucleotide from the support for the duration of the oligonucleotide deprotection and under the same conditions. |
| Deprotection: Deprotect using the protocol required by the nucleobases. |
| Storage: Controlled room temperature or lower, dry |
| Stability in Solution: Not Applicable |
chain terminators
In situations where ligation must be blocked at the 5’ terminus, 5’-OMe-dT may be used. 5’-OMe modification of a strand of siRNA using 5’-OMe-T can control guide strand selection and targeting specificity.1 5’-Amino-dT terminates an oligonucleotide with a 5’-amino group which may be used for attaching a peptide or a PNA sequence. To avoid polymerase extension at the 3’ terminus, 2’,3’-dideoxynucleoside and 3’-deoxynucleoside CPGs have proved to be effective. 2’,3’- Phosphoramidites are designed to be used with the 5’-phosphoramidites and supports. Since these phosphoramidites have no DMT group, they are not compatible with purification by the DMT-on technique. Ion exchange HPLC or PAGE should be used to purify these dideoxy terminated oligos to ensure that shorter sequences (containing 3’-OH) groups are removed. (3’-Termination can also be effected using a 3’-3’ linkage formed using 5’-supports, or 3’-spacer C3 CPG.)
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