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Oligo Synthesis

Oligo Synthesis : Supports, CPGs

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3'-Acridine CPG

3'-Acridine CPG

Glen Research

Catalogue No.DescriptionPack SizePriceQty
20-2973-013'-Acridine CPG 0.1g £109.00 Quantity Add to Order
20-2973-103'-Acridine CPG 1.0g £905.00 Quantity Add to Order
20-2973-133'-Acridine CPG 1x10µm £273.00 Quantity Add to Order
20-2973-143'-Acridine CPG 1x15µm £409.00 Quantity Add to Order
20-2973-413'-Acridine CPG 4x1.0µm £182.00 Quantity Add to Order
20-2973-423'-Acridine CPG 4x0.2µm £109.00 Quantity Add to Order

Description

3'-Acridine CPG

Structure

Catalog Number: 20-2973-xx

Description: 3'-Acridine CPG

1-Dimethoxytrityloxy-2-(N-acridinyl-4-aminobutyl)-propyl-3-O-succinoyl-
long chain alkylamino-CPG


F.W.: 450.86
Diluent: Not Applicable
Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group. (Acetyl-protected Cytidine should be used to avoid deamination when methanolic sodium hydroxide is used for deprotection.)
Deprotection: Cleavage and deprotection should be carried out using a mild deprotection: 0.4M methanolic sodium hydroxide (methanol:water 4:1) for 17 hours at room temperature. Pipet off support, neutralize with 1.5 volumes of 2M TEAA and desalt.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: Not Applicable

Acridine labelling

Acridine phosphoramidite is designed to produce an oligonucleotide containing acridine at any position in the molecule. Acridine CPG is used to label the 3’-terminus. Acridine is an effective intercalating agent.

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Protocols

MSDS

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Notes

QUESTION: Does AMA or methylamine cause any degradation to fluorescein or fluorescein-type dyes such as FAM or FITC?

RESPONSE:Response: While AMA (Ammonium hydroxide/40% Methylamine 1:1 v/v) is considered compatible with fluorescein, the use of methylamine when deprotecting a Fluorescein-labeled oligo does lead to a small amount of degradation, which is characterized by a the appearance of a late-eluting peak by RP HPLC that shows no visible fluorescein absorbance. With standard deprotection conditions (AMA 10 minutes at 65 C) the amount of degradation is approximately 5%. The impurity is not detected with AMA at RT for 2 hours.

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Related products

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