3'-Alkyne-Modifier Serinol CPG

Oligonucleotides prepared using 5’-Hexynyl Phosphoramidite are stable to standard deprotection conditions and exhibit a slightly increased retention time on RP HPLC. Azides are not compatible with oligonucleotide synthesis using phosphoramidites so a post-synthesis reaction is required. Azidobutyrate NHS Ester is used for azido-modification of amines at either the 3’-end or the 5’-end of an oligo and it can even be used for internal modification on an Amino-Modifier-C6 dX residue within the sequence. Specific to the 5’-terminus, 5’-Bromohexyl Phosphoramidite is added in the last cycle. This modifier can then be easily transformed into a 5’-azido group by displacement of bromide using sodium azide. Alkyne NHS ester allows the functionalization of an amino moiety in a variety of molecules, including DNA and RNA oligonucleotides as well as peptides or proteins. We also offer a synthesis support for labelling the 3’ terminus of oligonucleotides with an alkyne group for use in Click Chemistry. This builds upon our 1,3-diol product portfolio with the serinol backbone. A 5’-iodo-modified oligonucleotide (prepared using 5’-Iodo-dT) can be quantitatively converted to the corresponding 5’-azide.

 

Catalog Number: 20-2992-xx

Description: 3'-Alkyne-Modifier Serinol CPG

3-Dimethoxytrityloxy-2-(3-(3-propargyloxypropanamido)propanamido)propyl- 1-O-succinoyl long chain alkylamino CPG
		F.W.: 334.26

Diluent: n/a

Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.

Deprotection: Room temperature Ammonium Hydroxide as required by nucleobases.

Storage: Controlled room temperature or lower, dry

Stability in Solution: Not Applicable

Oligonucleotides prepared using 5’-Hexynyl Phosphoramidite are stable to standard deprotection conditions and exhibit a slightly increased retention time on RP HPLC. Azides are not compatible with oligonucleotide synthesis using phosphoramidites so a post-synthesis reaction is required. Azidobutyrate NHS Ester is used¹ for azido-modification of amines at either the 3’-end or the 5’-end of an oligo and it can even be used for internal modification on an Amino-Modifier-C6 dX residue within the sequence. Specific to the 5’-terminus, 5’-Bromohexyl Phosphoramidite is added in the last cycle. This modifier can then be easily transformed into a 5’-azido group by displacement of bromide using sodium azide.² Alkyne NHS ester allows the functionalization of an amino moiety in a variety of molecules, including DNA and RNA oligonucleotides as well as peptides or proteins. We also offer a synthesis support for labelling the 3’ terminus of oligonucleotides with an alkyne group for use in Click Chemistry. This builds upon our 1,3-diol product portfolio with the serinol backbone. A 5’-iodo-modified oligonucleotide (prepared using 5’-Iodo-dT) can be quantitatively converted to the corresponding 5’-azide.

 

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

 

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

(1) R. Kumar, et al., Journal of the American Chemical Society, 2007, 129, 6859-6864.
(2) J. Lietard, A. Meyer, J.J. Vasseur, and F. Morvan, Tetrahedron Letters, 2007, 48, 8795-8798.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

3'-Alkyne-Modifier Serinol CPG

3-Dimethoxytrityloxy-2-(3-(3-propargyloxypropanamido)propanamido)propyl- 1-O-succinoyl long chain alkylamino CPG
		F.W.: 334.26

Diluent: n/a

Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.

Deprotection: Room temperature Ammonium Hydroxide as required by nucleobases.

Storage: Controlled room temperature or lower, dry

Stability in Solution: Not Applicable

Material Safety Data Sheet

Literature Highlights

Glen Report 21.1: NEW PRODUCTS - CLICK CHEMISTRY, DMF-DG-5'-CE PHOSPHORAMIDITE

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

• View5'-Bromohexyl Phosphoramidite
• View5'-Hexynyl Phosphoramidite
• View5'-I-dT-CE Phosphoramidite
• ViewAlkyne-NHS Ester
• ViewAzidobutyrate NHS Ester