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Oligo Synthesis

Oligo Synthesis : CEPs

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Pyrrolo-C-TOM-CE Phosphoramidite

Pyrrolo-C-TOM-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-3017-02Pyrrolo-C-TOM-CE Phosphoramidite 0.25g £1,136.00 Quantity Add to Order
10-3017-90Pyrrolo-C-TOM-CE Phosphoramidite 100µmoles £450.00 Quantity Add to Order
10-3017-95Pyrrolo-C-TOM-CE Phosphoramidite 50µmoles £234.00 Quantity Add to Order

Description

Pyrrolo-C-TOM-CE Phosphoramidite

Structure

Catalog Number: 10-3017-xx

Description: Pyrrolo-C-TOM-CE Phosphoramidite

5'-Dimethoxytrityl-[6-methyl-pyrrolo-[2,3-d]-pyrimidine-2(3H)-one]-
2'-O-triisopropylsilyloxymethyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C52H72N5O9PSi M.W.: 970.23 F.W.: 343.27

Diluent: Anhydrous Acetonitrile
Coupling: 6 minute coupling time recommended
Deprotection: Ethanolic Ammonium Hydroxide (1:3) at room temperature for 24 hours or as required by the nucleobases. Remove silyl protecting group according to standard RNA procedures. Technical Bulletin
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 2-3 days
Please Note: This product is covered by US Patent No.: 7,144,995. Patented under European patent number 1483280.

MINOR RNA PHOSPHORAMIDITES (TOM PROTECTED)

Glen Research offers minor RNA phosphoramidites with either TOM or TBDMS protecting groups. 4-Thio-U, 5-Methyl-Cytidine, 2-Aminopurine riboside and 2-Amino-Adenosine are useful for analyzing RNA structure and activity relationships, for example, in ribozyme studies.

Pyrrolo-C is a fluorescent nucleoside whose fluorescence is sensitive to its environment and is ideal for probing RNA structure. It base-pairs as a normal C nucleotide. It is highly fluorescent and its excitation and emission are well to the red of most fluorescent nucleotide analogs, which eliminates or reduces background fluorescence from proteins. Pyrrolo-CTP has potential uses in biological assay development.

rSpacer is used to introduce an abasic site to an RNA sequence.

The protecting scheme for 2,6-Diaminopurine has been changed and the original product (10-3084) has been replaced with the optimized product (10-3085) below.

 

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Applications & Benefits

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-3017-02 0.25grams .25grams 2.58 72.67 43.6 27.25 19.82 14.53 3.63
10-3017-90 100µmoles .097grams 1 20 12 7.5 5.45 4 1
10-3017-95 50µmoles .049grams .5 3.33 2 1.25 .91 .67 .17
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-3017-02 0.25grams .25grams 3.85 .07 N/A N/A 14.71 N/A
10-3017-90 100µmoles .097grams 1.5 .07 N/A N/A 4.92 N/A
10-3017-95 50µmoles .049grams .75 .07 N/A N/A 1.79 N/A
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-3017-02 0.25grams .25grams 3.85 .07 N/A 11.65 10.94

10-3017-90 100µmoles .097grams 1.5 .07 N/A 4.06 3.82

10-3017-95 50µmoles .049grams .75 .07 N/A 1.65 1.55

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Related products

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200