Dependable, consistent high-fidelity PCR results with every target DNA template.

(Also a great replacement product for MasterAmp Extra-Long Read Kits - contact tech@cambio.co.uk for details)...

• Successful PCR, the first time and every time
• PCR amplification of difficult or high-GC templates (Fig. 1)
• Multiplex PCR compatible (Fig. 2)
• Robust amplification of targets up to 20 kb long
• High accuracy with 3-fold lower error rate than Taq DNA polymerase (error rate = 1 in 30,000)
• Extremely high sensitivity and specificity using the PCR Enhancer Technology (with Betaine)

The FailSafe™ PCR System provides dependable, consistent high-fidelity PCR results for every DNA template, regardless of its source or sequence. The FailSafe™ PCR System will faithfully amplify your template every time….We promise! Applications End-point PCR optimization Consistent and reproducible PCR How the FailSafe System Works First, purchase the FailSafe PCR PreMix Selection Kit (catalog number FS99060). The FailSafe PCR PreMix Selection Kit is composed of: FailSafe PCR Enzyme Blend (60 Units) Twelve (12) different FailSafe™ 2X PCR PreMixes. Each 2X PreMix contains dNTPs, Buffer and different concentrations of Mg and Epicentre's proprietary PCR Enhancer (with Betaine). Prepare and perform PCR reactions using your template and PCR primers with each of the 12 FailSafe™ 2X PCR PreMixes and the FailSafe PCR Enzyme. Analyze, by agarose gel electrophoresis, the PCR product produced from each of the 12 reactions and visually determine which PreMix gives the best amplification (e.g., a single, strong PCR product of the desired length or "as expected" multiplex PCR results). We guarantee that one of the FailSafe PCR 2X PreMixes will give the desired amplification product. For reliable and consistent PCR results, use the FailSafe PCR Enzyme and the best 2X PreMix identified in the optimization experiments for all subsequent amplifications with that template/primer combination. To reduce costs, purchase a FailSafe PCR System (Cat. No. FS99100, FS99250,or FS9901K) which provides sufficient enzyme and the appropriate amount of your favorite 2X PCR Premix(es) instead of buying the enzyme and 2X PCR Premixes separately.

Figure 1. Amplification of an 80%-85% GC-rich region of the human fragile X gene. PCR was performed using the FailSafe™ PCR System, schematically depicted above. Lanes A-L show the amplification products resulting from PCR using the 12 FailSafe PCR PreMixes. Lane M, molecular weight marker. In this experiment, optimal amplification was obtained with FailSafe PCR PreMix J. The size of the expected amplicon is indicated by an arrow.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Protocols for: FailSafe™ PCR System 

Due to the constant updating of the protocols by the manufacturer we have provided a direct link to Epicentre’s product page, where the latest protocol is available.

Please note this will open a new page or window on your computer.

Failsafe PCR PreMix Selection Kit / FailSafe™ PCR System / FailSafe™ Enzyme Mix Only / FailSafe™ PCR 2X PreMixes Protocol

(catalogue number FS99060 / FS99100 / FS99250 / FS9901K / FSE51100 / FSE5101K / FSP995A→L)

Please note: all protocols off site are the responsibility of the products supplier

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Customer endorsement

"EPICENTRE products are great, as for FailSafe™ PCR, we use it all the time."

Prof. Sir Martin Evans, FRS
Nobel Laureate, Cardiff University, Wales, UK

References:

1. Aiello, D., et al. (2010) Discovery and Characterization of Inhibitors of Pseudomonas aeruginosa Type III Secretion, Antimicrob. Agents Chemother. , AAC.01598-09.
2. Baskin, J. M., et al. (2010) Visualizing enveloping layer glycans during zebrafish early embryogenesis, PNAS 107 , 10360-10365.
3. Bazire, A., et al. (2010) The Sigma Factor AlgU Plays a Key Role in Formation of Robust Biofilms by Nonmucoid Pseudomonas aeruginosa, J. Bacteriol. 192 , 3001-3010.
4. Chen, S., et al. (2010) Molecular analysis of two bacterioferritin genes (bfr{alpha} and bfr{beta}) in the model rhizobacterium, Pseudomonas putida KT2440, Appl. Envir. Microbiol. , AEM.00215-10.
5. Chouljenko, V. N., et al. (2010) The Herpes Simplex Virus Type 1 UL20 Protein and the Amino Terminus of Glycoprotein K (gK) Physically Interact with gB, J. Virol. 84 , 8596-8606.
6. Chouljenko, V. N., et al. (2010) The Herpes Simplex Virus Type-1 (HSV-1) UL20 Protein and the Amino Terminus of Glycoprotein K (gK) Physically Interact with Glycoprotein B (gB), J. Virol. , JVI.00298-10.
7. Das, B., et al. (2010) Tissue Distribution of Murine Muc19/Smgc Gene Products, J. Histochem. Cytochem. 58 , 141-156.
8. Dusing, M. R., et al. (2010) Onecut-2 knockout mice fail to thrive during early postnatal period and have altered patterns of gene expression in small intestine, Physiol Genomics 42 , 115-125.
9. Grubman, A., et al. (2010) Vitamin B6 Is Required for Full Motility and Virulence in Helicobacter pylori, mBio 1 , e00112-10-11218.
10. Ho, P. A., et al. (2010) Prevalence and prognostic implications of WT1 mutations in pediatric acute myeloid leukemia (AML): a report from the Children's Oncology Group, Blood 116 , 702-710.
11. Hoehme, S., et al. (2010) Prediction and validation of cell alignment along microvessels as order principle to restore tissue architecture in liver regeneration, PNAS 107 , 10371-10376.
12. Ivey, F. D., et al. (2010) Activated Alleles of the Schizosaccharomyces pombe gpa2 G{alpha} Gene Identify Residues Involved in GDP-GTP Exchange, Eukaryot. Cell , EC.00010-10.
13. Kenney, M. C., et al. (2010) Characterization of Retinal and Blood Mitochondrial DNA from Age-Related Macular Degeneration Patients, Invest. Ophthalmol. Vis. Sci. 51 , 4289-4297.
14. Kier, B. L., et al. (2010) Stabilizing capping motif for {beta}-hairpins and sheets, PNAS 107 , 10466-10471.
15. Kim, D.-H., et al. (2010) Peptide Designed to Elicit Apoptosis in Adipose Tissue Endothelium Reduces Food Intake and Body Weight, Diabetes 59 , 907-915.
16. Krupenko, N. I., et al. (2010) ALDH1L2 Is the Mitochondrial Homolog of 10-Formyltetrahydrofolate Dehydrogenase, J. Biol. Chem. 285 , 23056-23063.
17. Li, B., et al. (2010) Catalytic promiscuity in the biosynthesis of cyclic peptide secondary metabolites in planktonic marine cyanobacteria, PNAS 107 , 10430-10435.
18. Lin, Y., et al. (2010) R loops stimulate genetic instability of CTG{middle dot}CAG repeats, PNAS 107 , 692-697.
19. McCarroll, J. A., et al. (2010) {beta}III-Tubulin Is a Multifunctional Protein Involved in Drug Sensitivity and Tumorigenesis in Non-Small Cell Lung Cancer, Cancer Res. 70 , 4995-5003.
20. Simeonova, D. D., et al. (2010) Identification and Heterologous Expression of Genes Involved in Anaerobic Dissimilatory Phosphite Oxidation by Desulfotignum phosphitoxidans, J. Bacteriol. , JB.00541-10.
21. Spradbery, P. (2010) Restriction fragment length polymorphisms of mutans streptococci in forensic odontological analysis, Bioscience Horizons 3 , 166-178.
22. Takai, K. K., et al. (2010) In Vivo Stoichiometry of Shelterin Components, J. Biol. Chem. 285 , 1457-1467.
23. Wang, G., et al. (2010) Process-based network decomposition reveals backbone motif structure, PNAS 107 , 10478-10483.
24. Bagwell, C. E., et al. (2009) Direct analysis of sulfate reducing bacterial communities in gas hydrate-impacted marine sediments by PCR-DGGE, J Basic Microbiol , .
25. Chan, L.-K., et al. (2009) Functional Analysis of Three Sulfide:Quinone Oxidoreductase Homologs in Chlorobaculum tepidum, J. Bacteriol. 191 , 1026-1034.
26. Chandler, J. R., et al. (2009) Mutational Analysis of Burkholderia thailandensis Quorum Sensing and Self-Aggregation, J. Bacteriol. 191 , 5901-5909.
27. Duerkop, B. A., et al. (2009) Quorum-Sensing Control of Antibiotic Synthesis in Burkholderia thailandensis, J. Bacteriol. 191 , 3909-3918.
28. Hamer, G. L., et al. (2009) Host Selection by Culex pipiens Mosquitoes and West Nile Virus Amplification, Am J Trop Med Hyg 80 , 268-278.
29. Hawkins, V. N., et al. (2009) Assessment of the Origins and Spread of Putative Resistance-Conferring Mutations in Plasmodium vivax Dihydropteroate Synthase, Am J Trop Med Hyg 81 , 348-355.
30. Klausen, C., et al. (2009) Cell Motility and Spreading Are Suppressed by HOXA4 in Ovarian Cancer Cells: Possible Involvement of {beta}1 Integrin, Mol. Cancer Res. 7 , 1425-1437.
31. Peychal, S. E. M., et al. (2009) Predominant modifier of extreme liver cancer susceptibility in C57BR/cdJ female mice localized to 6 Mb on chromosome 17, Carcinogenesis 30 , 879-885.
32. Shui, J., et al. (2009) Light-Dependent and Light-Independent Protochlorophyllide Oxidoreductases in the Chromatically Adapting Cyanobacterium Fremyella diplosiphon UTEX 481, Plant Cell Physiol. 50 , 1507-1521.
33. Spencer, W., et al. (2009) CtrA, a Global Response Regulator, Uses a Distinct Second Category of Weak DNA Binding Sites for Cell Cycle Transcription Control in Caulobacter crescentus, J. Bacteriol. 191 , 5458-5470.
34. Spencer, W., et al. (2009) CtrA, a global response regulator uses a distinct second category of weak DNA binding sites for cell-cycle transcription control in Caulobacter crescentus, J. Bacteriol. , JB.00355-09.
35. White, Y. A. R., et al. (2009) Targeted Gene Knockdown in Zebrafish Reveals Distinct Intraembryonic Functions for Insulin-Like Growth Factor II Signaling, Endocrinology 150 , 4366-4375.
36. Boyd, D. A., et al. (2008) Molecular Characterization of Enterococcus faecalis N06-0364 with Low-Level Vancomycin Resistance Harboring a Novel D-Ala-D-Ser Gene Cluster, vanL, Antimicrob. Agents Chemother. 52 , 2667-2672.
37. Boyd, D. A., et al. (2008) Salmonella Genomic Island 1 (SGI1), Variant SGI1-I, and New Variant SGI1-O in Proteus mirabilis Clinical and Food Isolates from China, Antimicrob. Agents Chemother. 52 , 340-344.
38. Degnan, P. H. & Moran, N. A. (2008) Diverse Phage-Encoded Toxins in a Protective Insect Endosymbiont, Appl. Envir. Microbiol. 74 , 6782-6791.
39. Imtiaz, M., et al. (2008) Characterization of Quantitative Trait Loci Controlling Genetic Variation for Preharvest Sprouting in Synthetic Backcross-Derived Wheat Lines, Genetics 178 , 1725-1736.
40. Keeney, D., et al. (2008) MarA-mediated overexpression of the AcrAB efflux pump results in decreased susceptibility to tigecycline in Escherichia coli, J. Antimicrob. Chemother. 61 , 46-53.
41. Liang, S., et al. (2008) A Pseudo-Full Mutation Identified in Fragile X Assay Reveals a Novel Base Change Abolishing an EcoRI Restriction Site, J. Mol. Diagn. 10 , 469-474.
42. Matsuoka, M., et al. (2008) A novel method for simple detection of mutations conferring drug resistance in Mycobacterium leprae, based on a DNA microarray, and its applicability in developing countries, J. Med. Microbiol. 57 , 1213-1219.
43. O'Connor, J. R., et al. (2008) Rifampin and Rifaximin Resistance in Clinical Isolates of Clostridium difficile, Antimicrob. Agents Chemother. 52 , 2813-2817.
44. Reeves, C. D., et al. (2008) Genes for the Biosynthesis of the Fungal Polyketides Hypothemycin from Hypomyces subiculosus and Radicicol from Pochonia chlamydosporia, Appl. Envir. Microbiol. 74 , 5121-5129.
45. Schilder, R. J., et al. (2008) Phase II Evaluation of Imatinib Mesylate in the Treatment of Recurrent or Persistent Epithelial Ovarian or Primary Peritoneal Carcinoma: A Gynecologic Oncology Group Study, J. Clin. Oncol. 26 , 3418-3425.
46. Thongdee, M., et al. (2008) Targeted Mutagenesis of Burkholderia thailandensis and Burkholderia pseudomallei through Natural Transformation of PCR Fragments, Appl. Envir. Microbiol. 74 , 2985-2989.
47. Xu, M., et al. (2008) NFX1 Interacts with mSin3A/Histone Deacetylase To Repress hTERT Transcription in Keratinocytes, Mol. Cell. Biol. 28 , 4819-4828.
48. Yuan, Y.-W. & Olmstead, R. G. (2008) Evolution and phylogenetic utility of the PHOT gene duplicates in the Verbena complex (Verbenaceae): dramatic intron size variation and footprint of ancestral recombination, Am. J. Botany 95 , 1166-1176.
49. Bakrania, P., et al. (2007) SOX2 anophthalmia syndrome: 12 new cases demonstrating broader phenotype and high frequency of large gene deletions, Br. J. Ophthalmol. 91 , 1471-1476.
50. Carter, G. P., et al. (2007) Binary Toxin Production in Clostridium difficile Is Regulated by CdtR, a LytTR Family Response Regulator, J. Bacteriol. 189 , 7290-7301.
51. Chen, S., et al. (2007) Mutational Analysis of the ompA Promoter from Flavobacterium johnsoniae, J. Bacteriol. 189 , 5108-5118.
52. Croal, L. R., et al. (2007) The fox Operon from Rhodobacter Strain SW2 Promotes Phototrophic Fe(II) Oxidation in Rhodobacter capsulatus SB1003, J. Bacteriol. 189 , 1774-1782.
53. Garcia-Rudaz, C., et al. (2007) Fxna, a novel gene differentially expressed in the rat ovary at the time of folliculogenesis, is required for normal ovarian histogenesis, Development 134 , 945-957.
54. Guan, X., et al. (2007) Mitochondrial DNA Sequence and Haplotype Variation Analysis in the Chicken (Gallus gallus), J. Hered. 98 , 723-726.
55. Lemos, D. R., et al. (2007) Evidence for Circadian Regulation of Activating Transcription Factor 5 But Not Tyrosine Hydroxylase by the Chromaffin Cell Clock, Endocrinology 148 , 5811-5821.
56. Milutinovic, S., et al. (2007) Valproate induces widespread epigenetic reprogramming which involves demethylation of specific genes, Carcinogenesis 28 , 560-571.
57. Naser, S. M., et al. (2007) Identification of lactobacilli by pheS and rpoA gene sequence analyses, Int J Syst Evol Microbiol 57 , 2777-2789.
58. O'Neill, A. J., et al. (2007) Genetic Basis of Resistance to Fusidic Acid in Staphylococci, Antimicrob. Agents Chemother. 51 , 1737-1740.
59. Schiffler, B., et al. (2007) Corynebacterium diphtheriae: Identification and Characterization of a Channel-Forming Protein in the Cell Wall, J. Bacteriol. 189 , 7709-7719.
60. van Berkum, P., et al. (2007) Chromosomal and Symbiotic Relationships of Rhizobia Nodulating Medicago truncatula and M. laciniata, Appl. Envir. Microbiol. 73 , 7597-7604.
61. Weaver, I. C. G., et al. (2007) The Transcription Factor Nerve Growth Factor-Inducible Protein A Mediates Epigenetic Programming: Altering Epigenetic Marks by Immediate-Early Genes, J. Neurosci. 27 , 1756-1768.
62. Cheung, B. B., et al. (2006) The Estrogen-responsive B Box Protein Is a Novel Regulator of the Retinoid Signal, J. Biol. Chem. 281 , 18246-18256.
63. Evsikov, A. V., et al. (2006) Cracking the egg: molecular dynamics and evolutionary aspects of the transition from the fully grown oocyte to embryo, Genes & Dev. 20 , 2713-2727.
64. Johnson, D. C., et al. (2006) Controlled Expression and Functional Analysis of Iron-Sulfur Cluster Biosynthetic Components within Azotobacter vinelandii, J. Bacteriol. 188 , 7551-7561.
65. Jones, C. H., et al. (2006) Identification and Sequence of a tet(M) Tetracycline Resistance Determinant Homologue in Clinical Isolates of Escherichia coli, J. Bacteriol. 188 , 7151-7164.
66. Jones, C. H., et al. (2006) Diagnostic PCR Analysis of the Occurrence of Methicillin and Tetracycline Resistance Genes among Staphylococcus aureus Isolates from Phase 3 Clinical Trials of Tigecycline for Complicated Skin and Skin Structure Infections, Antimicrob. Agents Chemother. 50 , 505-510.
67. Kao, R. S., et al. (2006) Schizosaccharomyces pombe Git1 Is a C2-Domain Protein Required for Glucose Activation of Adenylate Cyclase, Genetics 173 , 49-61.
68. Lepesheva, G. I., et al. (2006) CYP51 from Trypanosoma cruzi: A PHYLA-SPECIFIC RESIDUE IN THE B' HELIX DEFINES SUBSTRATE PREFERENCES OF STEROL 14{alpha}-DEMETHYLASE, J. Biol. Chem. 281 , 3577-3585.
69. Lin, K. C., et al. (2006) Candidate Gene Expression Analysis of Toxin-Induced Dilated Cardiomyopathy in the Turkey (Meleagris gallopavo), Poult. Sci. 85 , 2216-2221.
70. Ranger, B. S., et al. (2006) Globally Distributed Mycobacterial Fish Pathogens Produce a Novel Plasmid-Encoded Toxic Macrolide, Mycolactone F, Infect. Immun. 74 , 6037-6045.
71. Shao, G., et al. (2006) Epigenetic Inactivation of Betaig-h3 Gene in Human Cancer Cells, Cancer Res. 66 , 4566-4573.
72. Talebizadeh, Z., et al. (2006) Novel splice isoforms for NLGN3 and NLGN4 with possible implications in autism, J. Med. Genet. 43 , e21-.
73. van Berkum, P., et al. (2006) Multilocus Sequence Typing as an Approach for Population Analysis of Medicago-Nodulating Rhizobia, J. Bacteriol. 188 , 5570-5577.
74. Yeh, C.-T. & Yen, G.-C. (2006) Involvement of p38 MAPK and Nrf2 in phenolic acid-induced P-form phenol sulfotransferase expression in human hepatoma HepG2 cells, Carcinogenesis 27 , 1008-1017.
75. Zhao, Z., et al. (2006) Differential Binding of Cross-Reactive Anti-DNA Antibodies to Mesangial Cells: The Role of {alpha}-Actinin, J. Immunol. 176 , 7704-7714.
76. Das, A., et al. (2005) Cytochrome bd Oxidase, Oxidative Stress, and Dioxygen Tolerance of the Strictly Anaerobic Bacterium Moorella thermoacetica, J. Bacteriol. 187 , 2020-2029.
77. Gupta, S., et al. (2005) Cloning, expression, and functional characterization of cynomolgus monkey (Macaca fascicularis) CC chemokine receptor 1, J. Leukoc. Biol. 78 , 1175-1184.
78. Matsuda, T., et al. (2005) Silymarin Protects Pancreatic {beta}-Cells against Cytokine-Mediated Toxicity: Implication of c-Jun NH2-Terminal Kinase and Janus Kinase/Signal Transducer and Activator of Transcription Pathways, Endocrinology 146 , 175-185.
79. McAleese, F., et al. (2005) A Novel MATE Family Efflux Pump Contributes to the Reduced Susceptibility of Laboratory-Derived Staphylococcus aureus Mutants to Tigecycline, Antimicrob. Agents Chemother. 49 , 1865-1871.
80. Oshiro, N. & Pajor, A. M. (2005) Functional characterization of high-affinity Na /dicarboxylate cotransporter found in Xenopus laevis kidney and heart, Am J Physiol Cell Physiol 289 , C1159-1168.
81. Pickup, R. W., et al. (2005) Mycobacterium avium subsp. paratuberculosis in the Catchment Area and Water of the River Taff in South Wales, United Kingdom, and Its Potential Relationship to Clustering of Crohn's Disease Cases in the City of Cardiff, Appl. Envir. Microbiol. 71 , 2130-2139.
82. Tormay, P., et al. (2005) The Intercellular Signaling Activity of the Mycobacterium tuberculosis Chaperonin 60.1 Protein Resides in the Equatorial Domain, J. Biol. Chem. 280 , 14272-14277.
83. Weaver, I. C. G., et al. (2005) Reversal of Maternal Programming of Stress Responses in Adult Offspring through Methyl Supplementation: Altering Epigenetic Marking Later in Life, J. Neurosci. 25 , 11045-11054.
84. Zhang, L., et al. (2005) Diversity of Potential Short Tandem Repeats in Mycobacterium leprae and Application for Molecular Typing, J. Clin. Microbiol. 43 , 5221-5229.
85. Borovjagin, A. V. & Gerbi, S. A. (2004) Xenopus U3 snoRNA docks on pre-rRNA through a novel base-pairing interaction, RNA 10 , 942-953.
86. Brady, S. F., et al. (2004) Long-Chain N-Acyltyrosine Synthases from Environmental DNA, Appl. Envir. Microbiol. 70 , 6865-6870.
87. de Vries, W. N., et al. (2004) Maternal {beta}-catenin and E-cadherin in mouse development, Development 131 , 4435-4445.
88. Kenney, M. C., et al. (2004) MITOCHONDRIAL DNA IN HUMAN KERATOCONUS CORNEAS, Invest. Ophthalmol. Vis. Sci. 45 , 2886-.
89. Khare, S., et al. (2004) Rapid and Sensitive Detection of Mycobacterium avium subsp. paratuberculosis in Bovine Milk and Feces by a Combination of Immunomagnetic Bead Separation-Conventional PCR and Real-Time PCR, J. Clin. Microbiol. 42 , 1075-1081.
90. Lin-Su, K., et al. (2004) In Vitro Expression Studies of a Novel Mutation {Delta}299 in a Patient Affected with Apparent Mineralocorticoid Excess, J. Clin. Endocrinol. Metab. 89 , 2024-2027.
91. Ma, Y. & Hendershot, L. M. (2004) Herp Is Dually Regulated by Both the Endoplasmic Reticulum Stress-specific Branch of the Unfolded Protein Response and a Branch That Is Shared with Other Cellular Stress Pathways, J. Biol. Chem. 279 , 13792-13799.
92. Maier, T. M., et al. (2004) Construction and Characterization of a Highly Efficient Francisella Shuttle Plasmid, Appl. Envir. Microbiol. 70 , 7511-7519.
93. Matsuoka, M., et al. (2004) Genotyping of Mycobacterium leprae on the Basis of the Polymorphism of TTC Repeats for Analysis of Leprosy Transmission, J. Clin. Microbiol. 42 , 741-745.
94. Maxwell, P. H., et al. (2004) Ty1 Mobilizes Subtelomeric Y' Elements in Telomerase-Negative Saccharomyces cerevisiae Survivors, Mol. Cell. Biol. 24 , 9887-9898.
95. Milutinovic, S., et al. (2004) DNA Methyltransferase 1 Knock Down Induces Gene Expression by a Mechanism Independent of DNA Methylation and Histone Deacetylation, J. Biol. Chem. 279 , 27915-27927.
96. Moore, R. A., et al. (2004) Contribution of Gene Loss to the Pathogenic Evolution of Burkholderia pseudomallei and Burkholderia mallei, Infect. Immun. 72 , 4172-4187.
97. Pester, M., et al. (2004) pmoA-Based Analysis of Methanotrophs in a Littoral Lake Sediment Reveals a Diverse and Stable Community in a Dynamic Environment, Appl. Envir. Microbiol. 70 , 3138-3142.
98. Rishi, A. S., et al. (2004) Genome walking of large fragments: an improved method, J Biotechnol 111 , 9-15.
99. Sakakibara, S., et al. (2004) Accumulation of Heterochromatin Components on the Terminal Repeat Sequence of Kaposi's Sarcoma-Associated Herpesvirus Mediated by the Latency-Associated Nuclear Antigen, J. Virol. 78 , 7299-7310.
100. Stiefel, J., et al. (2004) Suppressors of an Adenylate Cyclase Deletion in the Fission Yeast Schizosaccharomyces pombe, Eukaryot. Cell 3 , 610-619.
101. Su, K., et al. (2004) A Promoter Haplotype of the Immunoreceptor Tyrosine-Based Inhibitory Motif-Bearing Fc{gamma}RIIb Alters Receptor Expression and Associates with Autoimmunity. II. Differential Binding of GATA4 and Yin-Yang1 Transcription Factors and Correlated Receptor Expression and Function, J. Immunol. 172 , 7192-7199.
102. Su, K., et al. (2004) A Promoter Haplotype of the Immunoreceptor Tyrosine-Based Inhibitory Motif-Bearing Fc{gamma}RIIb Alters Receptor Expression and Associates with Autoimmunity. I. Regulatory FCGR2B Polymorphisms and Their Association with Systemic Lupus Erythematosus, J. Immunol. 172 , 7186-7191.
103. Ulrich, R. L. (2004) Quorum Quenching: Enzymatic Disruption of N-Acylhomoserine Lactone-Mediated Bacterial Communication in Burkholderia thailandensis, Appl. Envir. Microbiol. 70 , 6173-6180.
104. Whitby, D., et al. (2004) Genotypic characterization of Kaposi's sarcoma-associated herpesvirus in asymptomatic infected subjects from isolated populations, J. Gen. Virol. 85 , 155-163.
105. Huang, X.-l., et al. (2003) Interferon-gamma Induces p11 Gene and Protein Expression in Human Epithelial Cells through Interferon-gamma -activated Sequences in the p11 Promoter, J. Biol. Chem. 278 , 9298-9308.
106. Julien, B. (2003) Characterization of the Integrase Gene and Attachment Site for the Myxococcus xanthus Bacteriophage Mx9, J. Bacteriol. 185 , 6325-6330.
107. Katafiasz, B. J., et al. (2003) Characterization of Cadherin-24, a Novel Alternatively Spliced Type II Cadherin, J. Biol. Chem. 278 , 27513-27519.
108. Meredith, T. C. & Woodard, R. W. (2003) Escherichia coli YrbH Is a D-Arabinose 5-Phosphate Isomerase, J. Biol. Chem. 278 , 32771-32777.
109. Salipante, S. J. & Hall, B. G. (2003) Determining the Limits of the Evolutionary Potential of an Antibiotic Resistance Gene, Mol. Biol. Evol. 20 , 653-659.
110. Salmassi, T. M. & Leadbetter, J. R. (2003) Analysis of genes of tetrahydrofolate-dependent metabolism from cultivated spirochaetes and the gut community of the termite Zootermopsis angusticollis, Microbiology 149 , 2529-2537.
111. Shan, H., et al. (2003) Preservation of motor neuron Ca2 channel sensitivity to insulin-like growth factor-1 in brain motor cortex from senescent rat, J. Physiol. 553 , 49-63.
112. Urbatsch, L. E., et al. (2003) Phylogenetic evaluation of Xylothamia, Gundlachia, and related genera (Asteraceae, Astereae) based on ETS and ITS nrDNA sequence data, Am. J. Botany 90 , 634-649.
113. Yoon, S.-R., et al. (2003) Huntington disease expansion mutations in humans can occur before meiosis is completed, PNAS 100 , 8834-8838.
114. Hahn, W. J. (2002) A Molecular Phylogenetic Study of the Palmae (Arecaceae) Based on atpB, rbcL, and 18S nrDNA Sequences, Syst Biol 51 , 92-112.
115. Pan, H., et al. (2002) Increased (CTG/CAG)n lengths in myotonic dystrophy type 1 and Machado-Joseph disease genes in idiopathic azoospermia patients, Hum. Reprod. 17 , 1578-1583.
116. Schadick, K., et al. (2002) Schizosaccharomyces pombe Git7p, a Member of the Saccharomyces cerevisiae Sgt1p Family, Is Required for Glucose and Cyclic AMP Signaling, Cell Wall Integrity, and Septation, Eukaryot. Cell 1 , 558-567.
117. Sun, S., et al. (2002) Identification and Characterization of the N-Acetylglucosamine Glycosyltransferase Gene of Haemophilus ducreyi, Infect. Immun. 70 , 5887-5892.
118. Eskra, L., et al. (2001) Brucella abortus Genes Identified following Constitutive Growth and Macrophage Infection, Infect. Immun. 69 , 7736-7742.
119. Forteza, R., et al. (2001) Hyaluronan serves a novel role in airway mucosal host defense, FASEB J 15 , 2179-2186.
120. Pajor, A. M., et al. (2001) Cloning and functional characterization of a high-affinity Na /dicarboxylate cotransporter from mouse brain, Am J Physiol Cell Physiol 280 , C1215-1223.
121. Scholes, D. T., et al. (2001) Multiple Regulators of Ty1 Transposition in Saccharomyces cerevisiae Have Conserved Roles in Genome Maintenance, Genetics 159 , 1449-1465.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Contains dNTPs, buffer, and various amounts of MgCl₂ and FailSafe™ PCR Enhancer (with betaine*)

* Patents issued and pending on FailSafe™ PCR Enhancer (with betaine)

FailSafe™ PCR Premix Selection Kits: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patent rights (such as 5´ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Applications

• Simplified PCR optimization.
• PCR of difficult or high-GC templates (Fig. 1).
• Multiplex PCR (Fig. 2).
• PCR amplifications up to 20 kb for cloning

Benefits

• Successful PCR, the first time and every time.
• High PCR accuracy, using a unique PCR Enzyme Mix with a 3-fold lower error rate than Taq DNA polymerase.
• Extremely high sensitivity and specificity using the PCR Enhancer Technology.
• FailSafe PCR PreMixes contain all components needed for successful PCR.
• Generates PCR products suitable for both TA cloning and blunt-end cloning.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

• ViewFailsafe™ Frequently Asked Questions
• ViewFailsafe™ and related Epicentre Products

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200