Cambio - Excellence in Molecular Biology

Aptamers

Aptamers: Catalogue Aptamers

Important information

Do you know what modifications you will need for your aptamers?  see "modifications" section for details

Fibronectin Aptamer to Human Protein Target

Fibronectin Aptamer to Human Protein Target (bovine plasma) ATW0008

Base Pair Bio

Catalogue No.DescriptionPack SizePriceQty
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ATW0008-100Fibronectin (bovine plasma) 10 AEU (100ug) POA Quantity Add to Order
ATW0008-25Fibronectin (bovine plasma) 2.5 AEU (25ug) POA Quantity Add to Order

Description

Fibronectin is a high-molecular weight (~440kDa) glycoprotein of the extracellular matrix that binds to membrane-spanning receptor proteins called integrins.  Similar to integrins, fibronectin binds extracellular matrix components such as collagen, fibrin, and heparan sulfate proteoglycans (e.g. syndecans).

This aptamer was selected by scientists at Base Pair Biotechnologies using proprietary methods and Fibronectin from bovine plasma, [Sigma Cat #F4759] but was also affinity tested with human plasma fibronectin, Advanced BioMatrix, San Diego, CA [Part No. 5050] as a target.  Both selection and validations done in 20 mM Tris, 100 mM NaCl, 0.005% Tween20 in nuclease free water, pH 7.4.  Let us know if you have any questions about its development or potential uses.

Target:   Fibronectin

Target Species:   bovine, human

Target Type:   protein

Affinity Constant (Kd):   1.7 nM

Default 3' Linker (Spacer) is 10T. Please submit a custom quote request for other options.

Aptamer information Kd 1.74nM

Datasheet

Please choose your modification and add as an extra catalogue number:
ATWUNMOD - Unmodified
ATWAMINE - Amine modification
ATWBIOTIN - Biotin modification
ATWTHIOL - Thiol modification (extra charge)
ATWFLUOR - Fluor (all) modification (extra charge)

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Protocols

Datasheet

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Notes

Antibody Equivalent Units (AEU). There is a  large molecular weight difference in antibodies (typically 150,000 Da) and Base Pair’s aptamers (~14,000 Da). We provide our aptamers in terms of moles, where the aptamer moles in 1  AEU is equal to antibody moles in the typical 100 microgram shipment from a leading antibody vendor. Therefore our standard 2.5 AEU (25ug) or 5 AEU (50ug) delivery is 2.5 and 5 times the equivalent moles of a standard antibody delivery of 100 microgram respectively.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Related products

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200