etect foodborne pathogens by semi-quantitative qPCR or conventional PCR with easy interpretable lateral flow evaluation.
Features
Target
• Salmonella enterica – invasion protein (invA) gene
• Yersinia enterocolitica – heat-stable enterotoxin A gene
• Shigella spp. – invasion plasmid antigen (ipaH6) gene
• Campylobacter spp. – acyl-[acyl-carrier-protein]--UDP-Nacetylglucosamine O-acyltransferase (lpxA) gene
• Clostridium perfringens – phospholipase C alpha toxin (plc) gene
• Shiga Toxin 1 – stx1 gene
• Shiga Toxin 2 – stx2 gene
• Escherichia coli O157 – wbdR gene
• Escherichia coli O104 – wckD gene
• Listeria spp. – invasion associated protein p60 (iap) gene
• Listeria monocytogenes – listeriolysin O (hly) gene
• Salmonella spp. – spacer-region between 16S and 23S RNA genes
Sensitivity
Down to 10 DNA copies/assay.
Principle
TaqMan® assay based on FAM and HEX labeled probes.
Content
qPCR Mix
Species Mix
Rehydration Buffer
PCR Grade Water
Internal Control
Positive Control
Sample Requirements
Isolated total DNA from potentially contaminated food serves here as starting material, typically after pre-cultivation of the sample growth medium.
Intended Use
For research use only!
Time to result
150 minutes
Cycler
• qTOWER (Analytik Jena)
• TOptical (Analytik Jena)
• Rotor-Gene® (Qiagen)
• Rotor-Gene®6000 (Qiagen)
• LightCycler® (Roche Diagnostics)
• Mastercycler® ep reaplex (Eppendorf)
• CFX Connect™ (Bio-Rad)
• StepOnePlus™, ABI 7500 (Applied Biosystem®)
• Mx3005P (Agilent Technologies)
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