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Cloning
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IG 5-alpha Chemically Competent Cells
|
Routine cloning and sub-cloning, library construction
|
≥1.0 × 109
|
|
IG 10B Chemically Competent Cells
|
Routine cloning and sub-cloning, library preparation
including large DNA >10 kb
|
≥1.0 × 1010
|
|
IG XL1 Blue Max Chemically Competent Cells
|
Stable plasmid propagation with blue/white screening
|
≥1.0 × 1010
|
|
HB101 Chemically Competent Cells
|
Stable plasmid maintenance
|
≥1.0 × 108
|
|
JM109(DE3) Chemically Competent Cells
|
Cloning and T7 protein expression strain
|
≥1.0 × 108
|
|
JM109 Chemically Competent Cells
|
Routine transformation and cloning
|
≥1.0 × 108
|
|
MG1655 Chemically Competent Cells
|
"Wildtype" E. coli strain
|
≥1.0 × 109
|
|
Specialized Cloning
|
IG Stable 2 Chemically Competent Cells
|
Cloning direct repeat sequences (unstable DNA)
|
≥1.0 × 109
|
|
IG Recombineering Chemically Competent Cells
|
Homologous recombination, DNA, plasmid, and BAC
engineering
|
≥1.0 × 109
|
|
IG ccdB Resist™ Chemically Competent Cells
|
Propagation of plasmids containing the ccdB gene
|
≥1.0 × 109
|
|
Protein Expression
|
IG Autoinduction DE3 Chemically Competent Cells
|
Optimized for autoinduction of protein expression
|
≥3.0 × 107
|
|
BL21(DE3) Chemically Competent Cells
|
Routine T7 protein expression
|
≥1.0 × 109
|
|
BL21 Chemically Competent Cells
|
Routine non-T7 protein expression
|
≥1.0 × 109
|
|
BL21(DE3)pLysS Chemically Competent Cells
|
T7 protein expression with tighter control for toxic
proteins
|
≥3.0 × 107
|
|
IG C41(DE3) Chemically Competent Cells
|
Improved expression of toxic proteins
|
≥3.0 × 107
|
|
IG C41(DE3)pLysS Chemically Competent Cells
|
Improved expression of toxic proteins with suppressed
basal expression
|
≥1.0 × 107
|
|
IG C43(DE3) Chemically Competent Cells
|
Most tolerant of toxic proteins
|
≥3.0 × 107
|
|
IG C43(DE3)pLysS Chemically Competent Cells
|
Most tolerant of toxic proteins with suppressed basal
expression
|
≥5.0 × 106
|
|
DL39(DE3) Chemically Competent Cells
|
Precise control over protein synthesis
|
≥1.0 × 107
|
|
Phage Display
|
ER2738 Phage Display Chemically Competent Cells
|
Preparation of antibody or peptide phage display libraries
|
≥1.0 × 1010
|
|
Medium
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IG Recovery Medium
|
Superior formulation for competent cell transformations
|
High-efficiency
|
|
IG Autoinduction Medium
|
Directly promotes high-yield growth of E. coli and
high-level expression of T7 promoter-regulated recombinant proteins
|
High-efficiency
|